{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T02:13:18Z","timestamp":1792462398115,"version":"4.5.0"},"reference-count":41,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2019,11,21]],"date-time":"2019-11-21T00:00:00Z","timestamp":1574294400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Megaprojects of Science Research for the 11th Five-Year Plan","award":["2007BAI18B02"],"award-info":[{"award-number":["2007BAI18B02"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"Objective<\/jats:title>To investigate the correlation between the single nucleotide polymorphisms (SNPs) in the toll-like receptor 4 (TLR4) gene and the susceptibility to chronic periodontitis.<\/jats:p><\/jats:sec>Design<\/jats:title>241 Chinese subjects from the cohort of Beijing Shijingshan Community were recruited. Buccal swab samples, the whole unstimulated saliva and periodontal clinical parameters were collected. Human DNA extracted from buccal swab samples were used for genotyping eight SNPs of the TLR4 gene (rs11536889<\/jats:ext-link>,rs1927906<\/jats:ext-link>,rs1927911<\/jats:ext-link>,rs2149356<\/jats:ext-link>,rs4986790<\/jats:ext-link>,rs4986791<\/jats:ext-link>,rs2737190<\/jats:ext-link>,rs787384<\/jats:ext-link>) by the Sequenom MassARRAY system.Porphyromonas gingivalis<\/jats:italic>(P. gingivalis<\/jats:italic>) was detected from the deposition of the whole unstimulated saliva through polymerase chain reaction (PCR) method based on 16S rRNA. The correlation between SNPs of TLR4 and chronic periodontitis susceptibility in the whole subjects and the patients detected withP.\u00a0gingivalis<\/jats:italic>was investigated.<\/jats:p><\/jats:sec>Results<\/jats:title>The variants ofrs4986790<\/jats:ext-link>andrs4986791<\/jats:ext-link>were not found in 241 Chinese subjects. Moreover, there was no significant difference in the distribution of theother6 SNPs of TLR4 between groups of none\/mild -periodontitis and moderate\/severe-periodontitis subjects. When combined withP. gingivalis<\/jats:italic>infection,rs1927911<\/jats:ext-link>(TT\/CC+CT),rs2149356<\/jats:ext-link>(TT\/GG+GT) andrs2737190<\/jats:ext-link>(GG\/AA+AG) were independent risk factors of chronic periodontitis.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Three SNPs of TLR4, i.e.,rs1927911<\/jats:ext-link>(TT\/CC+CT),rs2149356<\/jats:ext-link>(TT\/GG+GT) andrs2737190<\/jats:ext-link>(GG\/AA+AG), were associated with moderate\/severe chronic periodontitis in Chinese population infected withP. gingivalis<\/jats:italic>.P. gingivalis<\/jats:italic>, which interacted with TLR4 gene plays an important role in the pathogenesis of periodontitis.<\/jats:p><\/jats:sec>","DOI":"10.7717\/peerj.7828","type":"journal-article","created":{"date-parts":[[2019,11,21]],"date-time":"2019-11-21T10:11:27Z","timestamp":1574331087000},"page":"e7828","source":"Crossref","is-referenced-by-count":12,"title":["TLR4 polymorphisms may increase susceptibility to periodontitis in Pg-positive individuals"],"prefix":"10.7717","volume":"7","author":[{"given":"Wenjing","family":"Li","sequence":"first","affiliation":[{"name":"Department of Periodontology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Peking University School and Hospital of Stomatology, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojing","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Periodontology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Peking University School and Hospital of Stomatology, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"He","sequence":"additional","affiliation":[{"name":"Department of Periodontology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Peking University School and Hospital of Stomatology, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanxin","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Periodontology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Peking University School and Hospital of Stomatology, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingtao","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Periodontology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Peking University School and Hospital of Stomatology, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanting","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Periodontology, 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