{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:24:07Z","timestamp":1790745847624,"version":"4.1.0"},"reference-count":40,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2020,1,14]],"date-time":"2020-01-14T00:00:00Z","timestamp":1578960000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Plan Foundation of Jilin Province","award":["20160101008JC"],"award-info":[{"award-number":["20160101008JC"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Systemic sclerosis is a chronic multisystem autoimmune disease that is associated with polyclonal B cell hyperreactivity. The CDR3 of BCRs is the major site of antigen recognition. Therefore, we analyzed the BCR repertoire of patients with SSc. The BCR repertoires in 12 subjects including eight SSc patients and four healthy controls were characterized by high-throughput sequencing, and bioinformatics analysis were studied. The average CDR3 length in the SSc group was significantly shorter. The SSc patient displayed more diverse BCR. Moreover, SSc patients with mild skin sclerosis, anti-Scl70, interstitial lung disease or female sex were more diversified. B cells from the SSc patients showed a differential V and J gene usage. SSc patients had distinct BCR repertoires.These findings reflected the differences of BCR repertoires between SSc patients and controls. The higher-usage genes for the BCR sequence might be potential biomarkers of B cell-targeted therapies or diagnosis for SSc.<\/jats:p>","DOI":"10.7717\/peerj.8370","type":"journal-article","created":{"date-parts":[[2020,1,14]],"date-time":"2020-01-14T06:05:39Z","timestamp":1578981939000},"page":"e8370","source":"Crossref","is-referenced-by-count":18,"title":["An analysis of abnormalities in the B cell receptor repertoire in patients with systemic sclerosis using high-throughput sequencing"],"prefix":"10.7717","volume":"8","author":[{"given":"Xiaodong","family":"Shi","sequence":"first","affiliation":[{"name":"Rheumatology, First Hospital of Jilin University, Changchun, The People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tihong","family":"Shao","sequence":"additional","affiliation":[{"name":"Rheumatology, The First Affiliated Hospital of Anhui Medical University, Hefei, The People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feifei","family":"Huo","sequence":"additional","affiliation":[{"name":"Intensive Care Unit, First hospital of Jilin university, Changchun, The People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenqing","family":"Zheng","sequence":"additional","affiliation":[{"name":"Shenzhen RealOmics (Biotech) Co.Ltd, Shenzhen, The People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanyu","family":"Li","sequence":"additional","affiliation":[{"name":"Hepatology, First hospital of Jilin university, Changchun, The People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenyu","family":"Jiang","sequence":"additional","affiliation":[{"name":"Rheumatology, First Hospital of Jilin University, Changchun, The People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4443","published-online":{"date-parts":[[2020,1,14]]},"reference":[{"key":"10.7717\/peerj.8370\/ref-1","doi-asserted-by":"publisher","first-page":"1526","DOI":"10.1056\/NEJMoa021933","article-title":"Development of autoantibodies before the clinical onset of systemic lupus erythematosus","volume":"349","author":"Arbuckle","year":"2003","journal-title":"New England Journal of Medicine"},{"key":"10.7717\/peerj.8370\/ref-2","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1111\/imm.12927","article-title":"Antibody repertoire analysis in polygenic autoimmune diseases","volume":"155","author":"Bashford-Rogers","year":"2018","journal-title":"Immunology"},{"key":"10.7717\/peerj.8370\/ref-3","doi-asserted-by":"publisher","first-page":"2488","DOI":"10.1172\/JCI119433","article-title":"Analysis of the human VH gene repertoire. 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