{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T17:25:25Z","timestamp":1786296325434,"version":"build-2736575974"},"reference-count":36,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2020,6,19]],"date-time":"2020-06-19T00:00:00Z","timestamp":1592524800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"crossref","award":["2018YFC0910202 and 2016YFC1306300"],"award-info":[{"award-number":["2018YFC0910202 and 2016YFC1306300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004826","name":"Beijing Natural Science Foundation","doi-asserted-by":"crossref","award":["7172076 and 7192174"],"award-info":[{"award-number":["7172076 and 7192174"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Beijing cooperative construction project","award":["110651103"],"award-info":[{"award-number":["110651103"]}]},{"DOI":"10.13039\/501100002726","name":"Beijing Normal University","doi-asserted-by":"crossref","award":["11100704"],"award-info":[{"award-number":["11100704"]}],"id":[{"id":"10.13039\/501100002726","id-type":"DOI","asserted-by":"crossref"}]},{"name":"2016 PUMCH Science Fund for Junior Faculty","award":["pumch-2016-2.27"],"award-info":[{"award-number":["pumch-2016-2.27"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>To explore whether unilateral relapse of Bechet\u2019s disease-associated uveitis (BDU) causes differences in the tear proteome between the diseased and the contralateral quiescent eye and potential tear biomarkers for uveitis recurrence and disease monitoring.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>\n                      To minimize interindividual variations, bilateral tear samples were collected from the same patient (\n                      <jats:italic>n<\/jats:italic>\n                      = 15) with unilateral relapse of BDU. A data-independent acquisition (DIA) strategy was used to identify proteins that differed between active and quiescent eyes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 1,797 confident proteins were identified in the tear samples, of which 381 (21.2%) were also highly expressed in various tissues and organs. Fifty-one (2.8%) proteins differed in terms of expression between tears in active and quiescent eyes, 9 (17.6%) of which were functionally related to immunity or inflammation. Alpha-1-acid glycoprotein 1 (fold change = 3.2,\n                      <jats:italic>p<\/jats:italic>\n                      = 0.007) was increased and Annexin A1 (fold change = \u22121.7,\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.001) was decreased in the tears of the active BDU eye compared to the contralateral quiescent eye.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>A substantial amount of confident proteins were detected in the tears of BDU patients, including proteins that were deferentially expressed in the uveitis-relapsed eyes and the contralateral quiescent eyes. Some of these identified tear proteins play important roles in immune and inflammatory processes. Tear proteome might be a good source of biomarkers for uveitis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.7717\/peerj.9250","type":"journal-article","created":{"date-parts":[[2020,6,19]],"date-time":"2020-06-19T06:23:28Z","timestamp":1592547808000},"page":"e9250","source":"Crossref","is-referenced-by-count":19,"title":["Profiling tear proteomes of patients with unilateral relapsed Behcet\u2019s disease-associated uveitis using data-independent acquisition proteomics"],"prefix":"10.7717","volume":"8","author":[{"given":"Anyi","family":"Liang","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Qingdao Municipal Hospital, Qingdao University, Qingdao, China"},{"name":"Department of Biochemistry and 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