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The <jats:italic>Tex33<\/jats:italic> expression is found in cytoplasm of round spermatids in Mus musculus. However, the in vivo function of <jats:italic>Tex33<\/jats:italic> remains unknown. In this study, we made a 62bp in frame deletion on Exon2 of <jats:italic>Tex33<\/jats:italic> gene by CRISPR\/Cas9 in C57B\/L6 mouse, which cause frame shift mutation of <jats:italic>Tex33<\/jats:italic> gene. <jats:italic>Tex33<\/jats:italic><jats:sup>-\/-<\/jats:sup>adult male were fertile, and there is no significant change on litter size compared with male wildtype (<jats:italic>Tex33<\/jats:italic><jats:sup>+\/+<\/jats:sup>) adult. Besides, no overt differences were found in testis\/body weight ratios, testicular\/epididymal tissue morphology, sperm counts, sperm morphology and spermatozoa motility in adult <jats:italic>Tex33<jats:sup>-\/-<\/jats:sup><\/jats:italic>male mice (<jats:italic>N<\/jats:italic>\u00a0=\u00a03), in comparison with <jats:italic>Tex33<\/jats:italic><jats:sup>+\/+<\/jats:sup> adult (<jats:italic>N<\/jats:italic>\u00a0=\u00a03). TUNEL assay also indicates the germ cells apoptosis ratio was not significantly changed in adult <jats:italic>Tex33<jats:sup>-\/-<\/jats:sup><\/jats:italic> adult male mouse testis (<jats:italic>N<\/jats:italic>\u00a0=\u00a03), compared with adult <jats:italic>Tex33<jats:sup>+\/+<\/jats:sup><\/jats:italic> male (<jats:italic>N<\/jats:italic>\u00a0=\u00a03). Importantly, the first wave of elongating spermatids formation happens in 5w old mice. We find that the first wave of spermiogenesis is not disrupted in both 5-week-old <jats:italic>Tex33<jats:sup>+\/+<\/jats:sup><\/jats:italic> and <jats:italic>Tex33<\/jats:italic><jats:sup>-\/-<\/jats:sup>male mouse testes and three hallmarks of spermatogenesis, PLZF,<jats:italic>\u03b3<\/jats:italic>-H2AX and TNP1, are all detectable in seminiferous tubule. All results indicate that <jats:italic>Tex33<\/jats:italic> is a redundant gene to spermatogenesis. This study can help other researchers avoid repetitive works on redundant genes.<\/jats:p>","DOI":"10.7717\/peerj.9629","type":"journal-article","created":{"date-parts":[[2020,7,29]],"date-time":"2020-07-29T07:20:33Z","timestamp":1596007233000},"page":"e9629","source":"Crossref","is-referenced-by-count":14,"title":["Spermatogenesis is normal in <i>Tex33<\/i> knockout mice"],"prefix":"10.7717","volume":"8","author":[{"given":"Zhendong","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology, Nanjing Medical University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Nanjing Medical University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wentao","family":"Zeng","sequence":"additional","affiliation":[{"name":"Animal Core Facility of Nanjing Medical University, Nanjing Medical University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuqin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Animal Core Facility of Nanjing Medical University, Nanjing Medical University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianli","family":"Zhou","sequence":"additional","affiliation":[{"name":"Animal Core Facility of Nanjing Medical University, Nanjing Medical University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuomin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Nanjing Medical University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxi","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Nanjing Medical University, Nanjing, 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