{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T19:39:41Z","timestamp":1772307581782,"version":"3.50.1"},"reference-count":42,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T00:00:00Z","timestamp":1599177600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81773351"],"award-info":[{"award-number":["81773351"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["91749114"],"award-info":[{"award-number":["91749114"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81874251"],"award-info":[{"award-number":["81874251"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81703149"],"award-info":[{"award-number":["81703149"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81602789"],"award-info":[{"award-number":["81602789"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Platelet-rich plasma (PRP) has been reported recently as a potential therapeutic approach for alopecia, such as androgenetic alopecia, but the exact mechanisms and effects of specific components of this recipe remain largely unknown. In this study, we identified that platelet factor 4 (PF4), a component of PRP, significantly suppressed human hair follicle growth and restrained the proliferation of human dermal papilla cells (hDPCs). Furthermore, our results showed that PF4 upregulated androgen receptor (AR) in human dermal papilla cells <jats:italic>in vitro<\/jats:italic> and via hair follicle organ culture. Among the hair growth-promoting and DP-signature genes investigated, PF4 decreased the expression of <jats:italic>Wnt5a<\/jats:italic>, <jats:italic>Wnt10b<\/jats:italic>, <jats:italic>LEF1, HEY1<\/jats:italic> and <jats:italic>IGF-1,<\/jats:italic> and increased<jats:italic> DKK1<\/jats:italic> expression<jats:italic>,<\/jats:italic> but did not affect <jats:italic>BMP2<\/jats:italic> and <jats:italic>BMP4<\/jats:italic> expression. Collectively, Our data demonstrate that PF4 suppresses human hair follicle growth possibly via upregulating androgen receptor signaling and modulating hair growth-associated genes, which provides thought-provoking insights into the application and optimization of PRP in treating hair loss.<\/jats:p>","DOI":"10.7717\/peerj.9867","type":"journal-article","created":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T07:05:48Z","timestamp":1599203148000},"page":"e9867","source":"Crossref","is-referenced-by-count":8,"title":["Platelet factor 4 inhibits human hair follicle growth and promotes androgen receptor expression in human dermal papilla cells"],"prefix":"10.7717","volume":"8","author":[{"given":"Ke","family":"Sha","sequence":"first","affiliation":[{"name":"Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China"},{"name":"National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University,  Changsha, China"},{"name":"Key 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