{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T20:41:40Z","timestamp":1740170500375,"version":"3.37.3"},"reference-count":77,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T00:00:00Z","timestamp":1719878400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>The huhu beetle (<jats:italic>Prionoplus reticularis<\/jats:italic>) is the largest endemic beetle found throughout Aotearoa New Zealand, and is characterised by feeding on wood during its larval stage. It has been hypothesised that its gut microbiome plays a fundamental role in the degradation of wood. To explore this idea we examined the fungal and bacterial community composition of huhu grubs\u2019 frass, using amplicon sequencing. Grubs were reared on an exclusive diet of either a predominantly cellulose source (cotton) or lignocellulose source (pine) for 4 months; subsequently a diet switch was performed and the grubs were grown for another 4 months. The fungal community of cellulose-reared huhu grubs was abundant in potential cellulose degraders, contrasting with the community of lignocellulose-reared grubs, which showed abundant potential soft rot fungi, yeasts, and hemicellulose and cellulose degraders. Cellulose-reared grubs showed a less diverse fungal community, however, diet switch from cellulose to lignocellulose resulted in a change in community composition that showed grubs were still capable of utilising this substrate. Conversely, diet seemed to have a limited influence on huhu grub gut bacterial communities.<\/jats:p>","DOI":"10.7717\/peerj.17597","type":"journal-article","created":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T08:07:12Z","timestamp":1719907632000},"page":"e17597","source":"Crossref","is-referenced-by-count":0,"title":["Investigating the lignocellulolytic gut microbiome of huhu grubs (<i>Prionoplus reticularis<\/i>) using defined diets and dietary switch"],"prefix":"10.7717","volume":"12","author":[{"given":"Jay","family":"Viswam","sequence":"first","affiliation":[{"name":"Te Aka M\u0101tuatua-School of Science, Te Whare W\u0101nanga o Waikato-University of Waikato, Hamilton, Waikato, New Zealand"}]},{"given":"Mafalda","family":"Baptista","sequence":"additional","affiliation":[{"name":"Te Aka M\u0101tuatua-School of Science, Te Whare W\u0101nanga o Waikato-University of Waikato, Hamilton, Waikato, New Zealand"},{"name":"Waikato Regional Council, Hamilton, Waikato, New Zealand"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6562-4733","authenticated-orcid":true,"given":"Charles K.","family":"Lee","sequence":"additional","affiliation":[{"name":"Te Aka M\u0101tuatua-School of Science, Te Whare W\u0101nanga o Waikato-University of Waikato, Hamilton, Waikato, New Zealand"}]},{"given":"Hugh","family":"Morgan","sequence":"additional","affiliation":[{"name":"Te Aka M\u0101tuatua-School of Science, Te Whare W\u0101nanga o Waikato-University of Waikato, Hamilton, Waikato, New Zealand"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4847-6492","authenticated-orcid":true,"given":"Ian R.","family":"McDonald","sequence":"additional","affiliation":[{"name":"Te Aka M\u0101tuatua-School of Science, Te Whare W\u0101nanga o Waikato-University of Waikato, Hamilton, Waikato, New Zealand"}]}],"member":"4443","published-online":{"date-parts":[[2024,7,2]]},"reference":[{"key":"10.7717\/peerj.17597\/ref-1","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1111\/j.1469-8137.2009.03160.x","article-title":"The UNITE database for molecular identification of fungi-recent updates and future perspectives","volume":"186","author":"Abarenkov","year":"2010","journal-title":"The New Phytologist"},{"issue":"9","key":"10.7717\/peerj.17597\/ref-2","doi-asserted-by":"publisher","first-page":"1377","DOI":"10.1016\/j.jinsphys.2008.07.011","article-title":"Gut bacterial communities in the Mediterranean fruit fly (Ceratitis capitata) and their impact on host longevity","volume":"54","author":"Behar","year":"2008","journal-title":"Journal of Insect Physiology"},{"key":"10.7717\/peerj.17597\/ref-3","doi-asserted-by":"publisher","first-page":"159","DOI":"10.2436\/20.1501.01.246","article-title":"Functional symbiosis and communication in microbial ecosystems. 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