{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T09:07:47Z","timestamp":1772269667332,"version":"3.50.1"},"reference-count":99,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2018,5,28]],"date-time":"2018-05-28T00:00:00Z","timestamp":1527465600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"EU project Datos4.0","award":["C161046002"],"award-info":[{"award-number":["C161046002"]}]},{"name":"UPM"},{"DOI":"10.13039\/501100003759","name":"Universidad Polit\u00e9cnica de Madrid","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003759","id-type":"DOI","asserted-by":"crossref"}]},{"name":"KOPAR project","award":["655009"],"award-info":[{"award-number":["655009"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Experimental protocols are key when planning, performing and publishing research in many disciplines, especially in relation to the reporting of materials and methods. However, they vary in their content, structure and associated data elements. This article presents a guideline for describing key content for reporting experimental protocols in the domain of life sciences, together with the methodology followed in order to develop such guideline. As part of our work, we propose a checklist that contains 17 data elements that we consider fundamental to facilitate the execution of the protocol. These data elements are formally described in the SMART Protocols ontology. By providing guidance for the key content to be reported, we aim (1) to make it easier for authors to report experimental protocols with necessary and sufficient information that allow others to reproduce an experiment, (2) to promote consistency across laboratories by delivering an adaptable set of data elements, and (3) to make it easier for reviewers and editors to measure the quality of submitted manuscripts against an established criteria. Our checklist focuses on the content, what should be included. Rather than advocating a specific format for protocols in life sciences, the checklist includes a full description of the key data elements that facilitate the execution of the protocol.<\/jats:p>","DOI":"10.7717\/peerj.4795","type":"journal-article","created":{"date-parts":[[2018,5,28]],"date-time":"2018-05-28T03:22:31Z","timestamp":1527477751000},"page":"e4795","source":"Crossref","is-referenced-by-count":10,"title":["A guideline for reporting experimental protocols in life sciences"],"prefix":"10.7717","volume":"6","author":[{"given":"Olga","family":"Giraldo","sequence":"first","affiliation":[{"name":"Ontology Engineering Group, Campus de Montegancedo, Boadilla del Monte, Universidad Polit\u00e9cnica de Madrid, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Garcia","sequence":"additional","affiliation":[{"name":"Ontology Engineering Group, Campus de Montegancedo, Boadilla del Monte, Universidad Polit\u00e9cnica de Madrid, Madrid, Spain"},{"name":"Technische Universit\u00e4t Graz, Graz, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oscar","family":"Corcho","sequence":"additional","affiliation":[{"name":"Ontology Engineering Group, Campus de Montegancedo, Boadilla del Monte, Universidad Polit\u00e9cnica de Madrid, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4443","published-online":{"date-parts":[[2018,5,28]]},"reference":[{"key":"10.7717\/peerj.4795\/ref-1","article-title":"4TU. Centre for Research Data","author":"4TU","year":"2017"},{"issue":"Suppl 1","key":"10.7717\/peerj.4795\/ref-2","doi-asserted-by":"publisher","first-page":"S5","DOI":"10.1186\/2041-1480-5-S1-S5","article-title":"Evolving BioAssay Ontology (BAO): modularization, integration and applications","volume":"5","author":"Abeyruwan","year":"2014","journal-title":"Journal of Biomedical Semantics"},{"key":"10.7717\/peerj.4795\/ref-3","article-title":"Addgene: the nonprofit plasmid repository","author":"Addgene","year":"2018"},{"key":"10.7717\/peerj.4795\/ref-4","article-title":"Antibody Registry homepage","author":"Antibody Registry","year":"2018"},{"key":"10.7717\/peerj.4795\/ref-5","doi-asserted-by":"publisher","first-page":"6","DOI":"10.5334\/dsj-2016-006","article-title":"Are scientific data repositories coping with research data publishing?","volume":"15","author":"Assante","year":"2016","journal-title":"Data Science Journal"},{"issue":"7604","key":"10.7717\/peerj.4795\/ref-6","doi-asserted-by":"publisher","first-page":"452","DOI":"10.1038\/533452a","article-title":"1,500 scientists lift the lid on reproducibility","volume":"533","author":"Baker","year":"2016","journal-title":"Nature"},{"issue":"4","key":"10.7717\/peerj.4795\/ref-7","doi-asserted-by":"publisher","first-page":"e0154556","DOI":"10.1371\/journal.pone.0154556","article-title":"The ontology for biomedical investigations","volume":"11","author":"Bandrowski","year":"2016","journal-title":"PLOS ONE"},{"issue":"1","key":"10.7717\/peerj.4795\/ref-8","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1186\/1746-4811-7-12","article-title":"Agrobacterium-mediated transformation of safflower and the efficient recovery of transgenic plants via grafting","volume":"7","author":"Belide","year":"2011","journal-title":"Plant Methods"},{"key":"10.7717\/peerj.4795\/ref-9","article-title":"Plant Methods","author":"BioMed Central","year":"2017"},{"key":"10.7717\/peerj.4795\/ref-10","article-title":"Bio-protocol instructions for authors","author":"Bio-protocol","year":"2012"},{"key":"10.7717\/peerj.4795\/ref-11","doi-asserted-by":"publisher","first-page":"pdb.prot4688","DOI":"10.1101\/pdb.prot4688","article-title":"Quantitative GUS activity assay in intact plant tissue","volume":"2007","author":"Blazquez","year":"2007","journal-title":"Cold Spring Harbor Protocols"},{"issue":"23","key":"10.7717\/peerj.4795\/ref-12","doi-asserted-by":"publisher","first-page":"244","DOI":"10.21769\/BioProtoc.2623","article-title":"Determination of Mn concentrations in Synechocystis sp. PCC6803 using ICP-MS","volume":"7","author":"Brandenburg","year":"2002","journal-title":"Bio-protocol"},{"issue":"4","key":"10.7717\/peerj.4795\/ref-13","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments","volume":"55","author":"Bustin","year":"2009","journal-title":"Clinical Chemistry"},{"issue":"14","key":"10.7717\/peerj.4795\/ref-14","doi-asserted-by":"publisher","first-page":"e1188","DOI":"10.21769\/BioProtoc.1188","article-title":"Fluorescence microscopy for cilia in cultured cells and Zebrafish embryos","volume":"4","author":"Cao","year":"2014","journal-title":"Bio-protocol"},{"issue":"12","key":"10.7717\/peerj.4795\/ref-15","doi-asserted-by":"publisher","first-page":"4972","DOI":"10.1128\/IAI.00908-10","article-title":"Reproducible science","volume":"78","author":"Casadevall","year":"2010","journal-title":"Infection and Immunity"},{"key":"10.7717\/peerj.4795\/ref-16","article-title":"STAR methods author guide","author":"Cell Press","year":"2017"},{"issue":"8","key":"10.7717\/peerj.4795\/ref-17","doi-asserted-by":"publisher","first-page":"pdb.prot070599","DOI":"10.1101\/pdb.prot070599","article-title":"Labeling golgi with fluorescent ceramides","volume":"2012","author":"Chazotte","year":"2012","journal-title":"Cold Spring Harbor Protocols"},{"key":"10.7717\/peerj.4795\/ref-18","article-title":"Cold Spring Harbor protocols: instructions for authors","author":"Cold Spring Harbor Press","year":"2013"},{"key":"10.7717\/peerj.4795\/ref-19","article-title":"Commonwealth Scientific and Industrial Research Organisation data access portal","author":"Commonwealth Scientific and Industrial Research Organisation (CSIRO)","year":"2017"},{"issue":"3","key":"10.7717\/peerj.4795\/ref-20","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1007\/s12291-011-0110-x","article-title":"Validation protocol: first step of a lean-total quality management principle in a new laboratory set-up in a tertiary care hospital in India","volume":"26","author":"Das","year":"2011","journal-title":"Indian Journal of Clinical Biochemistry"},{"key":"10.7717\/peerj.4795\/ref-21","article-title":"Dryad homepage","author":"Dryad","year":"2017"},{"key":"10.7717\/peerj.4795\/ref-22","article-title":"What are the regulatory definitions for \u201cambient\u201d, \u201croom temperature\u201d and \u201ccold chain\u201d?","author":"ECA Foundation","year":"2017"},{"key":"10.7717\/peerj.4795\/ref-23","doi-asserted-by":"publisher","first-page":"D1086","DOI":"10.1093\/nar\/gku1127","article-title":"Type material in the NCBI taxonomy database","volume":"43","author":"Federhen","year":"2015","journal-title":"Nucleic Acids Research"},{"issue":"4","key":"10.7717\/peerj.4795\/ref-24","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1093\/ilar.43.4.244","article-title":"Guidelines for the design and statistical analysis of experiments using laboratory animals","volume":"43","author":"Festing","year":"2002","journal-title":"ILAR Journal"},{"key":"10.7717\/peerj.4795\/ref-25","article-title":"Figshare","author":"Figshare","year":"2017"},{"issue":"7","key":"10.7717\/peerj.4795\/ref-26","doi-asserted-by":"publisher","first-page":"e101131","DOI":"10.1371\/journal.pone.0101131","article-title":"The quality of methods reporting in parasitology experiments","volume":"9","author":"Fl\u00f3rez-Vargas","year":"2014","journal-title":"PLOS ONE"},{"key":"10.7717\/peerj.4795\/ref-27","article-title":"FDA guidance for industry update\u2014process validation","author":"Food and Drug Administration (FDA)","year":"2017"},{"key":"10.7717\/peerj.4795\/ref-28","article-title":"Resource identification initiative","author":"Force11","year":"2017"},{"key":"10.7717\/peerj.4795\/ref-29","doi-asserted-by":"publisher","first-page":"604","DOI":"10.12688\/f1000research.11334.1","article-title":"Reproducibility2020: progress and priorities","volume":"6","author":"Freedman","year":"2017","journal-title":"F1000Research"},{"key":"10.7717\/peerj.4795\/ref-30","doi-asserted-by":"publisher","DOI":"10.5281\/zenodo.1204838","article-title":"Corpus of protocols [Data set]","author":"Giraldo","year":"2018","journal-title":"Zenodo"},{"key":"10.7717\/peerj.4795\/ref-31","doi-asserted-by":"publisher","DOI":"10.5281\/zenodo.1204887","article-title":"Guidelines for reporting experimental protocols [Data set]","author":"Giraldo","year":"2018","journal-title":"Zenodo"},{"key":"10.7717\/peerj.4795\/ref-32","doi-asserted-by":"publisher","DOI":"10.5281\/zenodo.1204916","article-title":"Survey\u2014reporting an experimental protocol [Data set]","author":"Giraldo","year":"2018","journal-title":"Zenodo"},{"issue":"1","key":"10.7717\/peerj.4795\/ref-33","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1186\/s13326-017-0160-y","article-title":"Using semantics for representing experimental protocols","volume":"8","author":"Giraldo","year":"2017","journal-title":"Journal of Biomedical Semantics"},{"key":"10.7717\/peerj.4795\/ref-34","article-title":"GMR: Genetics and Molecular Research homepage","author":"GMR","year":"2017"},{"key":"10.7717\/peerj.4795\/ref-35","article-title":"DON\u2019T PUBLISH. RELEASE!.","author":"Goble","year":"2017"},{"key":"10.7717\/peerj.4795\/ref-36","doi-asserted-by":"publisher","DOI":"10.5281\/zenodo.1207846","article-title":"SMARTProtocols\/SMARTProtocols.github.io: first release of SMARTProtocols.github.io","author":"G\u00f3mez","year":"2018","journal-title":"Zenodo"},{"key":"10.7717\/peerj.4795\/ref-37","doi-asserted-by":"crossref","first-page":"D456","DOI":"10.1093\/nar\/gks1146","article-title":"The ChEBI reference database and ontology for biologically relevant chemistry: enhancements for 2013","volume":"41","author":"Hastings","year":"2013","journal-title":"Nucleic Acids Research"},{"key":"10.7717\/peerj.4795\/ref-38","article-title":"The Information Artifact Ontology (IAO)","author":"Information Artifact Ontology (IAO)","year":"2017"},{"key":"10.7717\/peerj.4795\/ref-39","article-title":"The Basic Formal Ontology (BFO)","author":"Institute for Formal Ontology and Medical Information Science (IFOMIS)","year":"2018"},{"key":"10.7717\/peerj.4795\/ref-40","article-title":"International Center for Tropical Agriculture (CIAT) homepage","author":"International Center for Tropical Agriculture (CIAT)","year":"2017"},{"key":"10.7717\/peerj.4795\/ref-41","doi-asserted-by":"publisher","first-page":"e3328","DOI":"10.3791\/3328","article-title":"In situ hybridization for the precise localization of transcripts in plants","volume":"57","author":"Javelle","year":"2011","journal-title":"Journal of Visualized Experiments"},{"key":"10.7717\/peerj.4795\/ref-42","article-title":"Instructions for authors","author":"Journal of Biological Methods (JBM)","year":"2013"},{"key":"10.7717\/peerj.4795\/ref-43","article-title":"Instructions for authors","author":"Journal of Visualized Experiments (JoVE)","year":"2012"},{"key":"10.7717\/peerj.4795\/ref-44","article-title":"Journal of Visualized Experiments","author":"Journal of Visualized Experiments (JoVE)","year":"2017"},{"key":"10.7717\/peerj.4795\/ref-45","doi-asserted-by":"publisher","first-page":"e1205","DOI":"10.3791\/1205","article-title":"Non-radioactive in situ hybridization protocol applicable for Norway spruce and a range of plant species","volume":"26","author":"Karlgren","year":"2009","journal-title":"Journal of Visualized Experiments"},{"issue":"6","key":"10.7717\/peerj.4795\/ref-46","doi-asserted-by":"publisher","first-page":"e1000412","DOI":"10.1371\/journal.pbio.1000412","article-title":"Improving bioscience research reporting: the ARRIVE guidelines for reporting animal research","volume":"8","author":"Kilkenny","year":"2010","journal-title":"PLOS Biology"},{"issue":"11","key":"10.7717\/peerj.4795\/ref-47","doi-asserted-by":"publisher","first-page":"e7824","DOI":"10.1371\/journal.pone.0007824","article-title":"Survey of the quality of experimental design, statistical analysis and reporting of research using animals","volume":"4","author":"Kilkenny","year":"2009","journal-title":"PLOS ONE"},{"issue":"11","key":"10.7717\/peerj.4795\/ref-48","doi-asserted-by":"publisher","first-page":"e7750","DOI":"10.1371\/journal.pone.0007750","article-title":"A rapid and economic in-house DNA purification method using glass syringe filters","volume":"4","author":"Kim","year":"2009","journal-title":"PLOS ONE"},{"issue":"D1","key":"10.7717\/peerj.4795\/ref-49","doi-asserted-by":"publisher","first-page":"D1202","DOI":"10.1093\/nar\/gkv951","article-title":"PubChem substance and compound databases","volume":"44","author":"Kim","year":"2016","journal-title":"Nucleic Acids Research"},{"key":"10.7717\/peerj.4795\/ref-50","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1177\/0049124107306660","article-title":"An introduction to the dataverse network as an infrastructure for data sharing","volume":"36","author":"King","year":"2007","journal-title":"Sociological Methods and Research"},{"issue":"3","key":"10.7717\/peerj.4795\/ref-51","doi-asserted-by":"publisher","first-page":"442","DOI":"10.1038\/nprot.2014.191","article-title":"Fluorescent in situ sequencing (FISSEQ) of RNA for gene expression profiling in intact cells and tissues","volume":"10","author":"Lee","year":"2015","journal-title":"Nature Protocols"},{"issue":"10","key":"10.7717\/peerj.4795\/ref-52","doi-asserted-by":"publisher","first-page":"926","DOI":"10.1002\/cyto.a.20623","article-title":"MIFlowCyt: the minimum information about a flow cytometry experiment","volume":"73A","author":"Lee","year":"2008","journal-title":"Cytometry Part A"},{"issue":"3","key":"10.7717\/peerj.4795\/ref-53","doi-asserted-by":"publisher","first-page":"655","DOI":"10.2144\/000113476","article-title":"Extraction of nucleic acids from yeast cells and plant tissues using ethanol as medium for sample preservation and cell disruption","volume":"49","author":"Linke","year":"2010","journal-title":"BioTechniques"},{"key":"10.7717\/peerj.4795\/ref-54","doi-asserted-by":"publisher","first-page":"e90","DOI":"10.21769\/BioProtoc.90","article-title":"Extract genomic DNA from arabidopsis leaves (can be used for other tissues as well)","volume":"Bio101","author":"Lu","year":"2011","journal-title":"Bio-protocol"},{"issue":"8","key":"10.7717\/peerj.4795\/ref-55","doi-asserted-by":"publisher","first-page":"1112","DOI":"10.1093\/bioinformatics\/btq099","article-title":"Modeling sample variables with an experimental factor ontology","volume":"26","author":"Malone","year":"2010","journal-title":"Bioinformatics"},{"issue":"5","key":"10.7717\/peerj.4795\/ref-56","doi-asserted-by":"publisher","first-page":"1059","DOI":"10.1016\/j.cell.2016.08.021","article-title":"A STAR is born","volume":"166","author":"Marcus","year":"2016","journal-title":"Cell"},{"key":"10.7717\/peerj.4795\/ref-57","doi-asserted-by":"publisher","first-page":"baw075","DOI":"10.1093\/database\/baw075","article-title":"Biosharing: curated and crowd-sourced metadata standards, databases and data policies in the life sciences","volume":"2016","author":"McQuilton","year":"2016","journal-title":"Database: The Journal of Biological Databases and Curation"},{"key":"10.7717\/peerj.4795\/ref-58","article-title":"Instructions for authors","author":"MethodsX","year":"2014"},{"key":"10.7717\/peerj.4795\/ref-59","article-title":"Minimum Information About a Cellular Assay (MIACA)","author":"MIACA","year":"2017"},{"key":"10.7717\/peerj.4795\/ref-60","article-title":"Minimum Information About a Plant Phenotyping Experiment (MIAPPE)","author":"MIAPPE","year":"2017"},{"key":"10.7717\/peerj.4795\/ref-61","article-title":"Minimum Information About an RNAi Experiment (MIARE)","author":"MIARE","year":"2017"},{"key":"10.7717\/peerj.4795\/ref-62","article-title":"Minimum information for biological and biomedical investigations","author":"Minimum Information for Biological and Biomedical Investigations (MIBBI)","year":"2017"},{"key":"10.7717\/peerj.4795\/ref-63","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1186\/s12916-015-0284-9","article-title":"The National Institutes of Health and guidance for reporting preclinical research","volume":"13","author":"Moher","year":"2015","journal-title":"BMC Medicine"},{"issue":"1","key":"10.7717\/peerj.4795\/ref-64","doi-asserted-by":"publisher","first-page":"R5","DOI":"10.1186\/gb-2012-13-1-r5","article-title":"Uberon, an integrative multi-species anatomy ontology","volume":"13","author":"Mungall","year":"2012","journal-title":"Genome Biology"},{"key":"10.7717\/peerj.4795\/ref-65","article-title":"Instructions for authors","author":"Nature Protocols","year":"2012"},{"key":"10.7717\/peerj.4795\/ref-66","article-title":"National institute of Dental and Craniofacial Research: version control guidelines","author":"National Institutes of Health (NIH)","year":"2017"},{"key":"10.7717\/peerj.4795\/ref-67","article-title":"The global unique device identification database","author":"National Institutes of Health (NIH)","year":"2018"},{"key":"10.7717\/peerj.4795\/ref-68","article-title":"Protocol exchange","author":"Nature Protocols Exchange (NPE)","year":"2017"},{"key":"10.7717\/peerj.4795\/ref-69","article-title":"CIMR: plant biology context Metabolomics Standards Initiative (MSI)","author":"Nikolau","year":"2006"},{"key":"10.7717\/peerj.4795\/ref-70","article-title":"ORCID \u2014connecting research and researchers","author":"ORCID","year":"2017"},{"key":"10.7717\/peerj.4795\/ref-71","article-title":"Plos One","author":"PLOS ONE","year":"2017"},{"key":"10.7717\/peerj.4795\/ref-72","article-title":"Open access repository of science methods","author":"protocols.io","year":"2018"},{"key":"10.7717\/peerj.4795\/ref-73","article-title":"ResearcherID","author":"ResearcherID","year":"2017"},{"key":"10.7717\/peerj.4795\/ref-74","article-title":"Resource identification portal","author":"RIP","year":"2018"},{"issue":"7","key":"10.7717\/peerj.4795\/ref-75","doi-asserted-by":"publisher","first-page":"1756","DOI":"10.1038\/nprot.2007.247","article-title":"Ancient DNA extraction from bones and teeth","volume":"2","author":"Rohland","year":"2007","journal-title":"Nature Protocols"},{"issue":"1","key":"10.7717\/peerj.4795\/ref-76","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1186\/1746-4811-7-4","article-title":"A simple and efficient method for isolating small RNAs from different plant species","volume":"7","author":"Rosas-C\u00e1rdenas","year":"2011","journal-title":"Plant Methods"},{"key":"10.7717\/peerj.4795\/ref-77","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1186\/2041-1480-5-37","article-title":"CLO: the cell line ontology","volume":"5","author":"Sarntivijai","year":"2014","journal-title":"Journal of Biomedical Semantics"},{"key":"10.7717\/peerj.4795\/ref-78","first-page":"25","article-title":"Cell line ontology: redesigning the cell line knowledgebase to aid integrative translational informatics","volume":"833","author":"Sarntivijai","year":"2011","journal-title":"Neoplasia"},{"issue":"3","key":"10.7717\/peerj.4795\/ref-79","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1145\/1084805.1084812","article-title":"A survey of data provenance in e-science","volume":"34","author":"Simmhan","year":"2005","journal-title":"ACM SIGMOD Record"},{"issue":"5","key":"10.7717\/peerj.4795\/ref-80","doi-asserted-by":"publisher","first-page":"R46","DOI":"10.1186\/gb-2005-6-5-r46","article-title":"Relations in biomedical ontologies","volume":"6","author":"Smith","year":"2005","journal-title":"Genome Biology"},{"issue":"13","key":"10.7717\/peerj.4795\/ref-81","doi-asserted-by":"publisher","first-page":"i295","DOI":"10.1093\/bioinformatics\/btn156","article-title":"The EXACT description of biomedical protocols","volume":"24","author":"Soldatova","year":"2008","journal-title":"Bioinformatics"},{"issue":"11","key":"10.7717\/peerj.4795\/ref-82","doi-asserted-by":"publisher","first-page":"795","DOI":"10.1098\/rsif.2006.0134","article-title":"An ontology of scientific experiments","volume":"3","author":"Soldatova","year":"2006","journal-title":"Journal of the Royal Society Interface"},{"issue":"Suppl 14","key":"10.7717\/peerj.4795\/ref-83","doi-asserted-by":"crossref","first-page":"S5","DOI":"10.1186\/1471-2105-15-S14-S5","article-title":"EXACT2: the semantics of biomedical protocols","volume":"15","author":"Soldatova","year":"2014","journal-title":"BMC Bioinformatics"},{"key":"10.7717\/peerj.4795\/ref-84","article-title":"Instructions for authors","author":"Springer Protocols","year":"2013"},{"issue":"8","key":"10.7717\/peerj.4795\/ref-85","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1371\/journal.pbio.1002538","article-title":"Protocols.io: virtual communities for protocol development and discussion","volume":"14","author":"Teytelman","year":"2016","journal-title":"PLOS Biology"},{"key":"10.7717\/peerj.4795\/ref-86","article-title":"Taxonomy database","author":"UniProt","year":"2017"},{"key":"10.7717\/peerj.4795\/ref-87","article-title":"Packaging and storage requirements","author":"United States Pharmacopeia (USP)","year":"2018"},{"issue":"1","key":"10.7717\/peerj.4795\/ref-88","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1186\/1746-4811-3-12","article-title":"Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs","volume":"3","author":"Varkonyi-Gasic","year":"2007","journal-title":"Plant Methods"},{"key":"10.7717\/peerj.4795\/ref-89","doi-asserted-by":"publisher","first-page":"e148","DOI":"10.7717\/peerj.148","article-title":"On the reproducibility of science: unique identification of research resources in the biomedical literature","volume":"1","author":"Vasilevsky","year":"2013","journal-title":"PeerJ"},{"issue":"3","key":"10.7717\/peerj.4795\/ref-90","doi-asserted-by":"publisher","first-page":"120","DOI":"10.2144\/000114263","article-title":"A single protocol for extraction of gDNA from bacteria and yeast","volume":"58","author":"Vingataramin","year":"2015","journal-title":"BioTechniques"},{"issue":"D1","key":"10.7717\/peerj.4795\/ref-91","doi-asserted-by":"publisher","first-page":"D955","DOI":"10.1093\/nar\/gkw1118","article-title":"PubChem BioAssay: 2017 update","volume":"45","author":"Wang","year":"2017","journal-title":"Nucleic Acids Research"},{"issue":"Web Server issue","key":"10.7717\/peerj.4795\/ref-92","doi-asserted-by":"publisher","first-page":"W541","DOI":"10.1093\/nar\/gkr469","article-title":"BioPortal: enhanced functionality via new Web services from the National Center for Biomedical Ontology to access and use ontologies in software applications","volume":"39","author":"Whetzel","year":"2011","journal-title":"Nucleic acids research"},{"key":"10.7717\/peerj.4795\/ref-93","article-title":"Current protocols: the fine art of experimentation. Instructions for authors","author":"Wiley\u2019s Current Protocols","year":"2012"},{"key":"10.7717\/peerj.4795\/ref-94","doi-asserted-by":"publisher","first-page":"160018","DOI":"10.1038\/sdata.2016.18","article-title":"The FAIR guiding principles for scientific data management and stewardship","volume":"3","author":"Wilkinson","year":"2016","journal-title":"Scientific Data"},{"key":"10.7717\/peerj.4795\/ref-95","article-title":"Guidelines for the storage of essential medicines and other health commodities","author":"World Health Organization (WHO)","year":"2003"},{"key":"10.7717\/peerj.4795\/ref-96","first-page":"279","article-title":"Ontobee: a linked data server and browser for ontology terms","author":"Xiang","year":"2011"},{"issue":"4","key":"10.7717\/peerj.4795\/ref-97","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1038\/nbt.3758","article-title":"Robotic crowd biology with maholo labdroids","volume":"35","author":"Yachie","year":"2017","journal-title":"Nature Biotechnology"},{"key":"10.7717\/peerj.4795\/ref-98","article-title":"Zenodo webpage","author":"Zenodo","year":"2017"},{"issue":"6","key":"10.7717\/peerj.4795\/ref-99","doi-asserted-by":"publisher","first-page":"pdb.prot5014","DOI":"10.1101\/pdb.prot5014","article-title":"Isolation and whole-cell patch clamping of arabidopsis guard cell protoplasts","volume":"2008","author":"Zhang","year":"2008","journal-title":"Cold Spring Harbor Protocols"}],"container-title":["PeerJ"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/peerj.com\/articles\/4795.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/peerj.com\/articles\/4795.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/peerj.com\/articles\/4795.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/peerj.com\/articles\/4795.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,5,28]],"date-time":"2018-05-28T03:22:50Z","timestamp":1527477770000},"score":1,"resource":{"primary":{"URL":"https:\/\/peerj.com\/articles\/4795"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5,28]]},"references-count":99,"alternative-id":["10.7717\/peerj.4795"],"URL":"https:\/\/doi.org\/10.7717\/peerj.4795","archive":["CLOCKSS","LOCKSS","Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.7287\/peerj.4795v0.1\/reviews\/3","asserted-by":"object"},{"id-type":"doi","id":"10.7287\/peerj.4795v0.1\/reviews\/2","asserted-by":"object"},{"id-type":"doi","id":"10.7287\/peerj.4795v0.1\/reviews\/1","asserted-by":"object"},{"id-type":"doi","id":"10.7287\/peerj.4795v0.2\/reviews\/1","asserted-by":"object"},{"id-type":"doi","id":"10.7287\/peerj.4795v0.2\/reviews\/4","asserted-by":"object"}]},"ISSN":["2167-8359"],"issn-type":[{"value":"2167-8359","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,5,28]]},"article-number":"e4795"}}