{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T15:43:22Z","timestamp":1768923802712,"version":"3.49.0"},"reference-count":17,"publisher":"Springer Science and Business Media LLC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,19]]},"DOI":"10.7759\/cureus.101876","type":"journal-article","created":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T00:22:37Z","timestamp":1768868557000},"source":"Crossref","is-referenced-by-count":0,"title":["Synergistic Enhancement of Non-viral SIRT1 Gene Delivery to Human Skin Equivalents Using Microneedling and Liposomal Vectors"],"prefix":"10.7759","author":[{"given":"Wataru","family":"Matsunaga","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takahiro","family":"Ishikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miki","family":"Nakashima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akinobu","family":"Gotoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.4161\/derm.22804","article-title":"Skin anti-aging strategies","volume":"4","author":"Ganceviciene R","year":"2012","unstructured":"Ganceviciene R, Liakou AI, Theodoridis A, Makrantonaki E, Zouboulis CC. Skin anti-aging strategies. Dermatoendocrinol. 2012, 4:308-19. 10.4161\/derm.22804","journal-title":"Dermatoendocrinol"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.3390\/cells13211799","article-title":"Novel approach to skin anti-aging: boosting pharmacological effects of exogenous nicotinamide adenine dinucleotide (NAD(+)) by synergistic inhibition of CD38 expression","volume":"13","author":"Kang S","year":"2024","unstructured":"Kang S, Park J, Cheng Z, Ye S, Jun SH, Kang NG. Novel approach to skin anti-aging: boosting pharmacological effects of exogenous nicotinamide adenine dinucleotide (NAD(+)) by synergistic inhibition of CD38 expression. Cells. 2024, 13:10.3390\/cells13211799","journal-title":"Cells"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.3390\/biom5020545","article-title":"Oxidative stress in aging human skin","volume":"5","author":"Rinnerthaler M","year":"2015","unstructured":"Rinnerthaler M, Bischof J, Streubel MK, Trost A, Richter K. Oxidative stress in aging human skin. Biomolecules. 2015, 5:545-89. 10.3390\/biom5020545","journal-title":"Biomolecules"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2014.04.002","article-title":"NAD+ and sirtuins in aging and disease","volume":"24","author":"Imai S","year":"2014","unstructured":"Imai S, Guarente L. NAD+ and sirtuins in aging and disease. Trends Cell Biol. 2014, 24:464-71. 10.1016\/j.tcb.2014.04.002","journal-title":"Trends Cell Biol"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2020.111215","article-title":"SIRT1 and aging related signaling pathways","volume":"187","author":"Chen C","year":"2020","unstructured":"Chen C, Zhou M, Ge Y, Wang X. SIRT1 and aging related signaling pathways. Mech Ageing Dev. 2020, 187:111215. 10.1016\/j.mad.2020.111215","journal-title":"Mech Ageing Dev"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.4c05945","article-title":"Enhanced SIRT1 activity by galangin mitigates UVB-Induced senescence in dermal fibroblasts via p53 acetylation regulation and activation","volume":"72","author":"Wen SY","year":"2024","unstructured":"Wen SY, Ng SC, Chiu YT, et al.. Enhanced SIRT1 activity by galangin mitigates UVB-Induced senescence in dermal fibroblasts via p53 acetylation regulation and activation. J Agric Food Chem. 2024, 72:23286-94. 10.1021\/acs.jafc.4c05945","journal-title":"J Agric Food Chem"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1007\/s40005-021-00512-4","article-title":"Microneedle for transdermal drug delivery: current trends and fabrication","volume":"51","author":"Jung JH","year":"2021","unstructured":"Jung JH, Jin SG. Microneedle for transdermal drug delivery: current trends and fabrication. J Pharm Investig. 2021, 51:503-17. 10.1007\/s40005-021-00512-4","journal-title":"J Pharm Investig"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.10.078","article-title":"Microneedles: a smart approach and increasing potential for transdermal drug delivery system","volume":"109","author":"Waghule T","year":"2019","unstructured":"Waghule T, Singhvi G, Dubey SK, Pandey MM, Gupta G, Singh M, Dua K. Microneedles: a smart approach and increasing potential for transdermal drug delivery system. Biomed Pharmacother. 2019, 109:1249-58. 10.1016\/j.biopha.2018.10.078","journal-title":"Biomed Pharmacother"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.22.10504","article-title":"Electroporation of mammalian skin: a mechanism to enhance transdermal drug delivery","volume":"90","author":"Prausnitz MR","year":"1993","unstructured":"Prausnitz MR, Bose VG, Langer R, Weaver JC. Electroporation of mammalian skin: a mechanism to enhance transdermal drug delivery. Proc Natl Acad Sci U S A. 1993, 90:10504-8. 10.1073\/pnas.90.22.10504","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/326497","article-title":"Liposomes for use in gene delivery","volume":"2011","author":"Balazs DA","year":"2011","unstructured":"Balazs DA, Godbey W. Liposomes for use in gene delivery. J Drug Deliv. 2011, 2011:326497. 10.1155\/2011\/326497","journal-title":"J Drug Deliv"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20092240","article-title":"Characterization of a new reconstructed full thickness skin model, T-Skin\u2122, and its application for investigations of anti-aging compounds","volume":"20","author":"Bataillon M","year":"2019","unstructured":"Bataillon M, Leli\u00e8vre D, Chapuis A, et al.. Characterization of a new reconstructed full thickness skin model, T-Skin\u2122, and its application for investigations of anti-aging compounds. Int J Mol Sci. 2019, 20:10.3390\/ijms20092240","journal-title":"Int J Mol Sci"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-04547-2","article-title":"Electroporation as a vaccine delivery system and a natural adjuvant to intradermal administration of plasmid DNA in macaques","volume":"7","author":"Todorova B","year":"2017","unstructured":"Todorova B, Adam L, Culina S, et al.. Electroporation as a vaccine delivery system and a natural adjuvant to intradermal administration of plasmid DNA in macaques. Sci Rep. 2017, 7:4122. 10.1038\/s41598-017-04547-2","journal-title":"Sci Rep"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2012.04.005","article-title":"Microneedle delivery of plasmid DNA to living human skin: formulation coating, skin insertion and gene expression","volume":"160","author":"Pearton M","year":"2012","unstructured":"Pearton M, Saller V, Coulman SA, Gateley C, Anstey AV, Zarnitsyn V, Birchall JC. Microneedle delivery of plasmid DNA to living human skin: formulation coating, skin insertion and gene expression. J Control Release. 2012, 160:561-9. 10.1016\/j.jconrel.2012.04.005","journal-title":"J Control Release"},{"key":"ref14","article-title":"Quantification of histochemical staining by color deconvolution","volume":"23","author":"Ruifrok A","year":"2001","unstructured":"Ruifrok A, Johnston D. Quantification of histochemical staining by color deconvolution. Anal Quant Cytol Histol. 2001, 23:291-9.","journal-title":"Anal Quant Cytol Histol"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118750","article-title":"Expression of naked DNA in human, pig, and mouse skin","volume":"97","author":"Hengge UR","year":"1996","unstructured":"Hengge UR, Walker PS, Vogel JC. Expression of naked DNA in human, pig, and mouse skin. J Clin Invest. 1996, 97:2911-6. 10.1172\/JCI118750","journal-title":"J Clin Invest"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1038\/12856","article-title":"Immunization via hair follicles by topical application of naked DNA to normal skin","volume":"17","author":"Fan H","year":"1999","unstructured":"Fan H, Lin Q, Morrissey GR, Khavari PA. Immunization via hair follicles by topical application of naked DNA to normal skin. Nat Biotechnol. 1999, 17:870-2. 10.1038\/12856","journal-title":"Nat Biotechnol"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1007\/s00417-019-04580-z","article-title":"SIRT1 is required for the neuroprotection of resveratrol on retinal ganglion cells after retinal ischemia-reperfusion injury in mice","volume":"258","author":"Luo J","year":"2020","unstructured":"Luo J, He T, Yang J, Yang N, Li Z, Xing Y. SIRT1 is required for the neuroprotection of resveratrol on retinal ganglion cells after retinal ischemia-reperfusion injury in mice. Graefes Arch Clin Exp Ophthalmol. 2020, 258:335-44. 10.1007\/s00417-019-04580-z","journal-title":"Graefes Arch Clin Exp Ophthalmol"}],"container-title":["Cureus"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cureus.com\/articles\/453410-synergistic-enhancement-of-non-viral-sirt1-gene-delivery-to-human-skin-equivalents-using-microneedling-and-liposomal-vectors","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T00:22:38Z","timestamp":1768868558000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cureus.com\/articles\/453410-synergistic-enhancement-of-non-viral-sirt1-gene-delivery-to-human-skin-equivalents-using-microneedling-and-liposomal-vectors"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,19]]},"references-count":17,"URL":"https:\/\/doi.org\/10.7759\/cureus.101876","relation":{},"ISSN":["2168-8184"],"issn-type":[{"value":"2168-8184","type":"print"}],"subject":[],"published":{"date-parts":[[2026,1,19]]}}}