{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T20:59:47Z","timestamp":1781038787624,"version":"3.54.1"},"reference-count":20,"publisher":"Springer Science and Business Media LLC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,9]]},"DOI":"10.7759\/cureus.110549","type":"journal-article","created":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T20:44:41Z","timestamp":1781037881000},"source":"Crossref","is-referenced-by-count":0,"title":["Association of Sperm Preparation and Fertilization Media Combinations With Fertilization Outcomes in Conventional In Vitro Fertilization: A Single-Center Retrospective Study"],"prefix":"10.7759","author":[{"given":"Mitsuru","family":"Nago","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manon","family":"Koyata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuria","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akari","family":"Saito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eri","family":"Kamioka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bunpei","family":"Ishizuka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2007.01036.x","article-title":"Long-time exposure of mouse embryos to the sperm produces high levels of reactive oxygen species in culture medium and relates to poor embryo development","volume":"44","author":"Enkhmaa D","year":"2009","unstructured":"Enkhmaa D, Kasai T, Hoshi K. Long-time exposure of mouse embryos to the sperm produces high levels of reactive oxygen species in culture medium and relates to poor embryo development. Reprod Domest Anim. 2009, 44:634-7. 10.1111\/j.1439-0531.2007.01036.x","journal-title":"Reprod Domest Anim"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.3109\/14647273.2013.859746","article-title":"Short co-incubation of gametes combined with early rescue ICSI: an optimal strategy for complete fertilization failure after IVF","volume":"17","author":"Liu W","year":"2014","unstructured":"Liu W, Liu J, Zhang X, Han W, Xiong S, Huang G. Short co-incubation of gametes combined with early rescue ICSI: an optimal strategy for complete fertilization failure after IVF. Hum Fertil (Camb). 2014, 17:50-5. 10.3109\/14647273.2013.859746","journal-title":"Hum Fertil (Camb)"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-008-9240-3","article-title":"Short gamete co-incubation during in vitro fertilization decreases the fertilization rate and does not improve embryo quality: a prospective auto controlled study","volume":"25","author":"Barraud-Lange V","year":"2008","unstructured":"Barraud-Lange V, Sifer C, Pocat\u00e9 K, et al.. Short gamete co-incubation during in vitro fertilization decreases the fertilization rate and does not improve embryo quality: a prospective auto controlled study. J Assist Reprod Genet. 2008, 25:305-10. 10.1007\/s10815-008-9240-3","journal-title":"J Assist Reprod Genet"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1186\/s12884-023-05490-z","article-title":"Brief and long co-incubation of sperm and oocytes for in vitro fertilization: a meta-analysis of randomized controlled trials","volume":"23","author":"Fan Y","year":"2023","unstructured":"Fan Y, Wu Z, Peng F, Peng H, Liang X, Zhu S. Brief and long co-incubation of sperm and oocytes for in vitro fertilization: a meta-analysis of randomized controlled trials. BMC Pregnancy Childbirth. 2023, 23:200. 10.1186\/s12884-023-05490-z","journal-title":"BMC Pregnancy Childbirth"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0132974","article-title":"The semen pH affects sperm motility and capacitation","volume":"10","author":"Zhou J","year":"2015","unstructured":"Zhou J, Chen L, Li J, et al.. The semen pH affects sperm motility and capacitation. PLoS One. 2015, 10:e0132974. 10.1371\/journal.pone.0132974","journal-title":"PLoS One"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.238816","article-title":"Capacitation-associated alkalization in human sperm is differentially controlled at the subcellular level","volume":"133","author":"Matamoros-Volante A","year":"2020","unstructured":"Matamoros-Volante A, Trevi\u00f1o CL. Capacitation-associated alkalization in human sperm is differentially controlled at the subcellular level. J Cell Sci. 2020, 133:jcs238816. 10.1242\/jcs.238816","journal-title":"J Cell Sci"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-020911-153258","article-title":"The control of male fertility by spermatozoan ion channels","volume":"74","author":"Lishko PV","year":"2012","unstructured":"Lishko PV, Kirichok Y, Ren D, Navarro B, Chung JJ, Clapham DE. The control of male fertility by spermatozoan ion channels. Annu Rev Physiol. 2012, 74:453-75. 10.1146\/annurev-physiol-020911-153258","journal-title":"Annu Rev Physiol"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2021.710887","article-title":"Physicochemical and functional characterization of female reproductive fluids: a report of the first two infants born following addition of their mother's fluids to the embryo culture media","volume":"12","author":"Canha-Gouveia A","year":"2021","unstructured":"Canha-Gouveia A, Prieto-S\u00e1nchez MT, S\u00e1nchez-Ferrer ML, et al.. Physicochemical and functional characterization of female reproductive fluids: a report of the first two infants born following addition of their mother's fluids to the embryo culture media. Front Physiol. 2021, 12:710887. 10.3389\/fphys.2021.710887","journal-title":"Front Physiol"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2001.tb02229.x","article-title":"The role of glucose in supporting motility and capacitation in human spermatozoa","volume":"22","author":"Williams AC","year":"2001","unstructured":"Williams AC, Ford WC. The role of glucose in supporting motility and capacitation in human spermatozoa. J Androl. 2001, 22:680-95. 10.1002\/j.1939-4640.2001.tb02229.x","journal-title":"J Androl"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1620\/tjem.165.99","article-title":"Importance of Ca2+, K+ and glucose in the medium for sperm penetration through the human zona pellucida","volume":"165","author":"Hoshi K","year":"1991","unstructured":"Hoshi K, Tsukikawa S, Sato A. Importance of Ca2+, K+ and glucose in the medium for sperm penetration through the human zona pellucida. Tohoku J Exp Med. 1991, 165:99-104. 10.1620\/tjem.165.99","journal-title":"Tohoku J Exp Med"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deae248","article-title":"The composition of commercially available human embryo culture media","volume":"40","author":"Zagers MS","year":"2025","unstructured":"Zagers MS, Laverde M, Goddijn M, de Groot JJ, Schrauwen FA, Vaz FM, Mastenbroek S. The composition of commercially available human embryo culture media. Hum Reprod. 2025, 40:30-40. 10.1093\/humrep\/deae248","journal-title":"Hum Reprod"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2024.104415","article-title":"Culture media with antioxidants improved preimplantation embryo development and clinical outcomes of patients of advanced age","volume":"50","author":"Mizumoto S","year":"2025","unstructured":"Mizumoto S, Nagao Y, Watanabe H, Tanaka K, Kuramoto T. Culture media with antioxidants improved preimplantation embryo development and clinical outcomes of patients of advanced age. Reprod Biomed Online. 2025, 50:104415. 10.1016\/j.rbmo.2024.104415","journal-title":"Reprod Biomed Online"},{"key":"ref13","article-title":"An Atlas of Human Gametes and Conceptuses: An Illustrated Reference for Assisted Reproductive Technology","author":"Veeck LL","year":"1999","unstructured":"Veeck LL. An Atlas of Human Gametes and Conceptuses: An Illustrated Reference for Assisted Reproductive Technology. Parthenon Publishing, New York; 1999."},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2012.244","article-title":"Investigation of the freely available easy-to-use software 'EZR' for medical statistics","volume":"48","author":"Kanda Y","year":"2013","unstructured":"Kanda Y. Investigation of the freely available easy-to-use software 'EZR' for medical statistics. Bone Marrow Transplant. 2013, 48:452-8. 10.1038\/bmt.2012.244","journal-title":"Bone Marrow Transplant"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.4103\/0974-1208.197691","article-title":"The quality of sperm preparation medium affects the motility, viability, and DNA integrity of human spermatozoa","volume":"9","author":"Anbari F","year":"2016","unstructured":"Anbari F, Halvaei I, Nabi A, Ghazali S, Khalili MA, Johansson L. The quality of sperm preparation medium affects the motility, viability, and DNA integrity of human spermatozoa. J Hum Reprod Sci. 2016, 9:254-8. 10.4103\/0974-1208.197691","journal-title":"J Hum Reprod Sci"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1055\/s-0035-1546423","article-title":"Optimal human embryo culture","volume":"33","author":"Swain JE","year":"2015","unstructured":"Swain JE. Optimal human embryo culture. Semin Reprod Med. 2015, 33:103-17. 10.1055\/s-0035-1546423","journal-title":"Semin Reprod Med"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmv034","article-title":"The effects of chemical and physical factors on mammalian embryo culture and their importance for the practice of assisted human reproduction","volume":"22","author":"Wale PL","year":"2016","unstructured":"Wale PL, Gardner DK. The effects of chemical and physical factors on mammalian embryo culture and their importance for the practice of assisted human reproduction. Hum Reprod Update. 2016, 22:2-22. 10.1093\/humupd\/dmv034","journal-title":"Hum Reprod Update"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2019.10.003","article-title":"'There is only one thing that is truly important in an IVF laboratory: everything' Cairo Consensus Guidelines on IVF Culture Conditions","volume":"40","author":"Consensus Group C","year":"2020","unstructured":"Consensus Group C. 'There is only one thing that is truly important in an IVF laboratory: everything' Cairo Consensus Guidelines on IVF Culture Conditions. Reprod Biomed Online. 2020, 40:33-60. 10.1016\/j.rbmo.2019.10.003","journal-title":"Reprod Biomed Online"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1093\/hropen\/hoz031","article-title":"The impact of selected embryo culture conditions on ART treatment cycle outcomes: a UK national study","volume":"2020","author":"Castillo CM","year":"2020","unstructured":"Castillo CM, Harper J, Roberts SA, O'Neill HC, Johnstone ED, Brison DR. The impact of selected embryo culture conditions on ART treatment cycle outcomes: a UK national study. Hum Reprod Open. 2020, 2020:hoz031. 10.1093\/hropen\/hoz031","journal-title":"Hum Reprod Open"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-023-02934-5","article-title":"Culture conditions in the IVF laboratory: state of the ART and possible new directions","volume":"40","author":"Sciorio R","year":"2023","unstructured":"Sciorio R, Rinaudo P. Culture conditions in the IVF laboratory: state of the ART and possible new directions. J Assist Reprod Genet. 2023, 40:2591-607. 10.1007\/s10815-023-02934-5","journal-title":"J Assist Reprod Genet"}],"container-title":["Cureus"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cureus.com\/articles\/494434-association-of-sperm-preparation-and-fertilization-media-combinations-with-fertilization-outcomes-in-conventional-in-vitro-fertilization-a-single-center-retrospective-study","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T20:44:42Z","timestamp":1781037882000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cureus.com\/articles\/494434-association-of-sperm-preparation-and-fertilization-media-combinations-with-fertilization-outcomes-in-conventional-in-vitro-fertilization-a-single-center-retrospective-study"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,9]]},"references-count":20,"URL":"https:\/\/doi.org\/10.7759\/cureus.110549","relation":{},"ISSN":["2168-8184"],"issn-type":[{"value":"2168-8184","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6,9]]}}}