{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T17:58:01Z","timestamp":1781287081781,"version":"3.54.1"},"reference-count":22,"publisher":"Springer Science and Business Media LLC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,12]]},"DOI":"10.7759\/cureus.110747","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T17:23:06Z","timestamp":1781284986000},"source":"Crossref","is-referenced-by-count":0,"title":["Benchmark Comparison of Bone Marrow Aspiration Systems: Increased Progenitor Cell Concentrations Achieved Without Centrifugation Using a Novel Filtration Design With High Surface Area"],"prefix":"10.7759","author":[{"given":"Anthony","family":"Scillia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teo","family":"Mendez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Kuebler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bob","family":"Salvat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-199711000-00012","article-title":"Aspiration to obtain osteoblast progenitor cells from human bone marrow: the influence of aspiration volume","volume":"79","author":"Muschler GF","year":"1997","unstructured":"Muschler GF, Boehm C, Easley K. Aspiration to obtain osteoblast progenitor cells from human bone marrow: the influence of aspiration volume. J Bone Joint Surg Am. 1997, 79:1699-709. 10.2106\/00004623-199711000-00012","journal-title":"J Bone Joint Surg Am"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1007\/s00264-013-2017-z","article-title":"Benefits of small volume and small syringe for bone marrow aspirations of mesenchymal stem cells","volume":"37","author":"Hernigou P","year":"2013","unstructured":"Hernigou P, Homma Y, Flouzat Lachaniette CH, Poignard A, Allain J, Chevallier N, Rouard H. Benefits of small volume and small syringe for bone marrow aspirations of mesenchymal stem cells. Int Orthop. 2013, 37:2279-87. 10.1007\/s00264-013-2017-z","journal-title":"Int Orthop"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-018-1750-x","article-title":"Isolation of clinically relevant concentrations of bone marrow mesenchymal stem cells without centrifugation","volume":"17","author":"Scarpone M","year":"2019","unstructured":"Scarpone M, Kuebler D, Chambers A, et al.. Isolation of clinically relevant concentrations of bone marrow mesenchymal stem cells without centrifugation. J Transl Med. 2019, 17:10. 10.1186\/s12967-018-1750-x","journal-title":"J Transl Med"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.D.02215","article-title":"Percutaneous autologous bone-marrow grafting for nonunions. Influence of the number and concentration of progenitor cells","volume":"87","author":"Hernigou P","year":"2005","unstructured":"Hernigou P, Poignard A, Beaujean F, Rouard H. Percutaneous autologous bone-marrow grafting for nonunions. Influence of the number and concentration of progenitor cells. J Bone Joint Surg Am. 2005, 87:1430-7. 10.2106\/JBJS.D.02215","journal-title":"J Bone Joint Surg Am"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0203714","article-title":"How stem cell composition in bone marrow aspirate relates to clinical outcomes when used for cervical spine fusion","volume":"13","author":"Chaput CD","year":"2018","unstructured":"Chaput CD, Shar A, Jupiter D, Hubert Z, Clough B, Krause U, Gregory CA. How stem cell composition in bone marrow aspirate relates to clinical outcomes when used for cervical spine fusion. PLoS One. 2018, 13:e0203714. 10.1371\/journal.pone.0203714","journal-title":"PLoS One"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1186\/s12891-015-0714-z","article-title":"A dose response analysis of a specific bone marrow concentrate treatment protocol for knee osteoarthritis","volume":"16","author":"Centeno CJ","year":"2015","unstructured":"Centeno CJ, Al-Sayegh H, Bashir J, Goodyear S, Freeman MD. A dose response analysis of a specific bone marrow concentrate treatment protocol for knee osteoarthritis. BMC Musculoskelet Disord. 2015, 16:258. 10.1186\/s12891-015-0714-z","journal-title":"BMC Musculoskelet Disord"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5411.143","article-title":"Multilineage potential of adult human mesenchymal stem cells","volume":"284","author":"Pittenger MF","year":"1999","unstructured":"Pittenger MF, Mackay AM, Beck SC, et al.. Multilineage potential of adult human mesenchymal stem cells. Science. 1999, 284:143-7. 10.1126\/science.284.5411.143","journal-title":"Science"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.L.01529","article-title":"Quantitative assessment of the yield of osteoblastic connective tissue progenitors in bone marrow aspirate from the iliac crest, tibia, and calcaneus","volume":"95","author":"Hyer CF","year":"2013","unstructured":"Hyer CF, Berlet GC, Bussewitz BW, Hankins T, Ziegler HL, Philbin TM. Quantitative assessment of the yield of osteoblastic connective tissue progenitors in bone marrow aspirate from the iliac crest, tibia, and calcaneus. J Bone Joint Surg Am. 2013, 95:1312-6. 10.2106\/JBJS.L.01529","journal-title":"J Bone Joint Surg Am"},{"key":"ref9","article-title":"Relationship between differing volumes of bone marrow aspirates and their cellular composition","volume":"6","author":"Batini\u0107 D","year":"1990","unstructured":"Batini\u0107 D, Marusi\u0107 M, Pavleti\u0107 Z, Bogdani\u0107 V, Uzarevi\u0107 B, Nemet D, Labar B. Relationship between differing volumes of bone marrow aspirates and their cellular composition. Bone Marrow Transplant. 1990, 6:103-7.","journal-title":"Bone Marrow Transplant"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.00458","article-title":"Human mesenchymal stromal cell (MSC) characteristics vary among laboratories when manufactured from the same source material: a report by the Cellular Therapy Team of the Biomedical Excellence for Safer Transfusion (BEST) Collaborative","volume":"8","author":"Stroncek DF","year":"2020","unstructured":"Stroncek DF, Jin P, McKenna DH, et al.. Human mesenchymal stromal cell (MSC) characteristics vary among laboratories when manufactured from the same source material: a report by the Cellular Therapy Team of the Biomedical Excellence for Safer Transfusion (BEST) Collaborative. Front Cell Dev Biol. 2020, 8:458. 10.3389\/fcell.2020.00458","journal-title":"Front Cell Dev Biol"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00025.2005","article-title":"The bone marrow vascular niche: home of HSC differentiation and mobilization","volume":"20","author":"Kopp HG","year":"2005","unstructured":"Kopp HG, Avecilla ST, Hooper AT, Rafii S. The bone marrow vascular niche: home of HSC differentiation and mobilization. Physiology (Bethesda). 2005, 20:349-56. 10.1152\/physiol.00025.2005","journal-title":"Physiology (Bethesda)"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1007\/s10439-008-9577-x","article-title":"The mechanical environment of bone marrow: a review","volume":"36","author":"Gurkan UA","year":"2008","unstructured":"Gurkan UA, Akkus O. The mechanical environment of bone marrow: a review. Ann Biomed Eng. 2008, 36:1978-91. 10.1007\/s10439-008-9577-x","journal-title":"Ann Biomed Eng"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0236946","article-title":"Impact of side-hole geometry on the performance of hemodialysis catheter tips: a computational fluid dynamics assessment","volume":"15","author":"Owen DG","year":"2020","unstructured":"Owen DG, de Oliveira DC, Qian S, Green NC, Shepherd DE, Espino DM. Impact of side-hole geometry on the performance of hemodialysis catheter tips: a computational fluid dynamics assessment. PLoS One. 2020, 15:e0236946. 10.1371\/journal.pone.0236946","journal-title":"PLoS One"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1007\/s00167-016-3981-9","article-title":"Bone marrow concentrate and platelet-rich plasma differ in cell distribution and interleukin 1 receptor antagonist protein concentration","volume":"26","author":"Cassano JM","year":"2018","unstructured":"Cassano JM, Kennedy JG, Ross KA, Fraser EJ, Goodale MB, Fortier LA. Bone marrow concentrate and platelet-rich plasma differ in cell distribution and interleukin 1 receptor antagonist protein concentration. Knee Surg Sports Traumatol Arthrosc. 2018, 26:333-42. 10.1007\/s00167-016-3981-9","journal-title":"Knee Surg Sports Traumatol Arthrosc"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.24166","article-title":"Functional heterogeneity of mesenchymal stem cells: implications for cell therapy","volume":"113","author":"Phinney DG","year":"2012","unstructured":"Phinney DG. Functional heterogeneity of mesenchymal stem cells: implications for cell therapy. J Cell Biochem. 2012, 113:2806-12. 10.1002\/jcb.24166","journal-title":"J Cell Biochem"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-019-1866-7","article-title":"Quantitation of progenitor cell populations and growth factors after bone marrow aspirate concentration","volume":"17","author":"Sch\u00e4fer R","year":"2019","unstructured":"Sch\u00e4fer R, DeBaun MR, Fleck E, et al.. Quantitation of progenitor cell populations and growth factors after bone marrow aspirate concentration. J Transl Med. 2019, 17:115. 10.1186\/s12967-019-1866-7","journal-title":"J Transl Med"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-83241-w","article-title":"Selective loss of microvesicles is a major issue of the differential centrifugation isolation protocols","volume":"11","author":"Nigro A","year":"2021","unstructured":"Nigro A, Finardi A, Ferraro MM, Manno DE, Quattrini A, Furlan R, Romano A. Selective loss of microvesicles is a major issue of the differential centrifugation isolation protocols. Sci Rep. 2021, 11:3589. 10.1038\/s41598-021-83241-w","journal-title":"Sci Rep"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1007\/s43465-024-01194-4","article-title":"Autologous bone marrow aspiration concentrate (BMAC) therapy for primary knee osteoarthritis \u2014 an observational and dose escalation study","volume":"58","author":"Jeyaraman M","year":"2024","unstructured":"Jeyaraman M, Karthik KS, Choudary D, Jeyaraman N, Nallakumarasamy A, Ramasubramian S. Autologous bone marrow aspiration concentrate (BMAC) therapy for primary knee osteoarthritis \u2014 an observational and dose escalation study. Indian J Orthop. 2024, 58:1016-26. 10.1007\/s43465-024-01194-4","journal-title":"Indian J Orthop"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1177\/2325967117724398","article-title":"Single- versus multiple-site harvesting techniques for bone marrow concentrate: evaluation of aspirate quality and pain","volume":"5","author":"Oliver K","year":"2017","unstructured":"Oliver K, Awan T, Bayes M. Single- versus multiple-site harvesting techniques for bone marrow concentrate: evaluation of aspirate quality and pain. Orthop J Sports Med. 2017, 5:2325967117724398. 10.1177\/2325967117724398","journal-title":"Orthop J Sports Med"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1001\/jamasurg.2017.6233","article-title":"Understanding costs of care in the operating room","volume":"153","author":"Childers CP","year":"2018","unstructured":"Childers CP, Maggard-Gibbons M. Understanding costs of care in the operating room. JAMA Surg. 2018, 153:e176233. 10.1001\/jamasurg.2017.6233","journal-title":"JAMA Surg"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1080\/14653240902923161","article-title":"Comparison of two single-platform ISHAGE-based CD34 enumeration protocols on BD FACSCalibur and FACSCanto flow cytometers","volume":"11","author":"Sutherland DR","year":"2009","unstructured":"Sutherland DR, Nayyar R, Acton E, Giftakis A, Dean S, Mosiman VL. Comparison of two single-platform ISHAGE-based CD34 enumeration protocols on BD FACSCalibur and FACSCanto flow cytometers. Cytotherapy. 2009, 11:595-605. 10.1080\/14653240902923161","journal-title":"Cytotherapy"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1089\/scd.1.1996.5.213","article-title":"The ISHAGE guidelines for CD34+ cell determination by flow cytometry","volume":"5","author":"Sutherland DR","year":"1996","unstructured":"Sutherland DR, Anderson L, Keeney M, Nayar R, Chin-Yee I. The ISHAGE guidelines for CD34+ cell determination by flow cytometry. J Hematother. 1996, 5:213-26. 10.1089\/scd.1.1996.5.213","journal-title":"J Hematother"}],"container-title":["Cureus"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cureus.com\/articles\/489605-benchmark-comparison-of-bone-marrow-aspiration-systems-increased-progenitor-cell-concentrations-achieved-without-centrifugation-using-a-novel-filtration-design-with-high-surface-area","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T17:23:08Z","timestamp":1781284988000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cureus.com\/articles\/489605-benchmark-comparison-of-bone-marrow-aspiration-systems-increased-progenitor-cell-concentrations-achieved-without-centrifugation-using-a-novel-filtration-design-with-high-surface-area"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,12]]},"references-count":22,"URL":"https:\/\/doi.org\/10.7759\/cureus.110747","relation":{},"ISSN":["2168-8184"],"issn-type":[{"value":"2168-8184","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6,12]]}}}