{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T15:49:42Z","timestamp":1784044182462,"version":"3.55.0"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,4,26]]},"DOI":"10.7759\/cureus.24516","type":"journal-article","created":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T05:37:28Z","timestamp":1651037848000},"source":"Crossref","is-referenced-by-count":2,"title":["Comparison of the Fetal Fraction of Cell-Free DNA in In-Vitro Fertilization (IVF) Versus Natural Conception Evaluation of the Fetal Fraction With IVF Parameters"],"prefix":"10.7759","author":[{"given":"Kostas","family":"Kallianidis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evangelia","family":"Dimitroulia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Depy","family":"Mavrogianni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuaela","family":"Liokari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ritsa","family":"Bletsa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elli","family":"Anagnostou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikos","family":"Sofikitis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitrios","family":"Loutradis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1210\/js.2016-1052","article-title":"Proteomics of children born after intracytoplasmic sperm injection reveal indices of an adverse cardiometabolic profile","volume":"1","author":"Kosteria I","year":"2017","unstructured":"Kosteria I, Tsangaris GT, Gkourogianni A, et al.. Proteomics of children born after intracytoplasmic sperm injection reveal indices of an adverse cardiometabolic profile. J Endocr Soc. 2017, 1:288-301. 10.1210\/js.2016-1052","journal-title":"J Endocr Soc"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0094001","article-title":"Plasma metabolomic profiling suggests early indications for predisposition to latent insulin resistance in children conceived by ICSI","volume":"9","author":"Gkourogianni A","year":"2014","unstructured":"Gkourogianni A, Kosteria I, Telonis AG, et al.. Plasma metabolomic profiling suggests early indications for predisposition to latent insulin resistance in children conceived by ICSI. PLoS One. 2014, 9:e94001. 10.1371\/journal.pone.0094001","journal-title":"PLoS One"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1907472116","article-title":"Noninvasive preimplantation genetic testing for aneuploidy in spent medium may be more reliable than trophectoderm biopsy","volume":"116","author":"Huang L","year":"2019","unstructured":"Huang L, Bogale B, Tang Y, Lu S, Xie XS, Racowsky C. Noninvasive preimplantation genetic testing for aneuploidy in spent medium may be more reliable than trophectoderm biopsy. Proc Natl Acad Sci U S A. 2019, 116:14105-12. 10.1073\/pnas.1907472116","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1613294113","article-title":"Noninvasive chromosome screening of human embryos by genome sequencing of embryo culture medium for in vitro fertilization","volume":"113","author":"Xu J","year":"2016","unstructured":"Xu J, Fang R, Chen L, et al.. Noninvasive chromosome screening of human embryos by genome sequencing of embryo culture medium for in vitro fertilization. Proc Natl Acad Sci U S A. 2016, 113:11907-12. 10.1073\/pnas.1613294113","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref5","doi-asserted-by":"crossref","DOI":"10.1093\/clinchem\/48.2.386","article-title":"Correlation of fetal DNA and human chorionic gonadotropin concentrations in second-trimester maternal serum","volume":"48","author":"Ohashi Y","year":"2002","unstructured":"Ohashi Y, Miharu N, Honda H, Samura O, Ohama K. Correlation of fetal DNA and human chorionic gonadotropin concentrations in second-trimester maternal serum. Clin Chem. 2002, 48:386-8.","journal-title":"Clin Chem"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2004.12.015","article-title":"Placental volume, as measured by 3-dimensional sonography and levels of maternal plasma cell-free fetal DNA","volume":"193","author":"Wataganara T","year":"2005","unstructured":"Wataganara T, Metzenbauer M, Peter I, Johnson KL, Bianchi DW. Placental volume, as measured by 3-dimensional sonography and levels of maternal plasma cell-free fetal DNA. Am J Obstet Gynecol. 2005, 193:496-500. 10.1016\/j.ajog.2004.12.015","journal-title":"Am J Obstet Gynecol"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2014.03.042","article-title":"Noninvasive prenatal screening for fetal trisomies 21, 18, 13 and the common sex chromosome aneuploidies from maternal blood using massively parallel genomic sequencing of DNA","volume":"211","author":"Porreco RP","year":"2014","unstructured":"Porreco RP, Garite TJ, Maurel K, et al.. Noninvasive prenatal screening for fetal trisomies 21, 18, 13 and the common sex chromosome aneuploidies from maternal blood using massively parallel genomic sequencing of DNA. Am J Obstet Gynecol. 2014, 211:365.e1-12. 10.1016\/j.ajog.2014.03.042","journal-title":"Am J Obstet Gynecol"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1002\/uog.15851","article-title":"Screening for trisomies by cell-free DNA testing of maternal blood: consequences of a failed result","volume":"47","author":"Revello R","year":"2016","unstructured":"Revello R, Sarno L, Ispas A, Akolekar R, Nicolaides KH. Screening for trisomies by cell-free DNA testing of maternal blood: consequences of a failed result. Ultrasound Obstet Gynecol. 2016, 47:698-704. 10.1002\/uog.15851","journal-title":"Ultrasound Obstet Gynecol"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1159\/000337373","article-title":"Fetal fraction in maternal plasma cell-free DNA at 11-13 weeks' gestation: effect of maternal and fetal factors","volume":"31","author":"Ashoor G","year":"2012","unstructured":"Ashoor G, Poon L, Syngelaki A, Mosimann B, Nicolaides KH. Fetal fraction in maternal plasma cell-free DNA at 11-13 weeks' gestation: effect of maternal and fetal factors. Fetal Diagn Ther. 2012, 31:237-43. 10.1159\/000337373","journal-title":"Fetal Diagn Ther"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2012.01.029","article-title":"Chromosome-selective sequencing of maternal plasma cell-free DNA for first-trimester detection of trisomy 21 and trisomy 18","volume":"206","author":"Ashoor G","year":"2012","unstructured":"Ashoor G, Syngelaki A, Wagner M, Birdir C, Nicolaides KH. Chromosome-selective sequencing of maternal plasma cell-free DNA for first-trimester detection of trisomy 21 and trisomy 18. Am J Obstet Gynecol. 2012, 206:322.e1-5. 10.1016\/j.ajog.2012.01.029","journal-title":"Am J Obstet Gynecol"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1002\/uog.12331","article-title":"Fetal fraction in maternal plasma cell-free DNA at 11-13 weeks' gestation: relation to maternal and fetal characteristics","volume":"41","author":"Ashoor G","year":"2013","unstructured":"Ashoor G, Syngelaki A, Poon LC, Rezende JC, Nicolaides KH. Fetal fraction in maternal plasma cell-free DNA at 11-13 weeks' gestation: relation to maternal and fetal characteristics. Ultrasound Obstet Gynecol. 2013, 41:26-32. 10.1002\/uog.12331","journal-title":"Ultrasound Obstet Gynecol"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dey033","article-title":"Cell-free fetal DNA testing in singleton IVF conceptions","volume":"33","author":"Lee TJ","year":"2018","unstructured":"Lee TJ, Rolnik DL, Menezes MA, McLennan AC, da Silva Costa F. Cell-free fetal DNA testing in singleton IVF conceptions. Hum Reprod. 2018, 33:572-8. 10.1093\/humrep\/dey033","journal-title":"Hum Reprod"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1086\/301800","article-title":"Quantitative analysis of fetal DNA in maternal plasma and serum: implications for noninvasive prenatal diagnosis","volume":"62","author":"Lo YM","year":"1998","unstructured":"Lo YM, Tein MS, Lau TK, et al.. Quantitative analysis of fetal DNA in maternal plasma and serum: implications for noninvasive prenatal diagnosis. Am J Hum Genet. 1998, 62:768-75. 10.1086\/301800","journal-title":"Am J Hum Genet"},{"key":"ref14","article-title":"Telomerase RNA as a detection marker in the serum of breast cancer patients","volume":"6","author":"Chen XQ","year":"2000","unstructured":"Chen XQ, Bonnefoi H, Pelte MF, et al.. Telomerase RNA as a detection marker in the serum of breast cancer patients. Clin Cancer Res. 2000, 6:3823-6.","journal-title":"Clin Cancer Res"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016\/s0009-8981(02)00091-8","article-title":"Cell-free DNA: measurement in various carcinomas and establishment of normal reference range","volume":"321","author":"Wu TL","year":"2002","unstructured":"Wu TL, Zhang D, Chia JH, Tsao K-, Sun CF, Wu JT. Cell-free DNA: measurement in various carcinomas and establishment of normal reference range. Clin Chim Acta. 2002, 321:77-87. 10.1016\/s0009-8981(02)00091-8","journal-title":"Clin Chim Acta"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.2174\/138920112799361891","article-title":"ESR1, ESR2 and FSH receptor gene polymorphisms in combination: a useful genetic tool for the prediction of poor responders","volume":"13","author":"Anagnostou E","year":"2012","unstructured":"Anagnostou E, Mavrogianni D, Theofanakis C, et al.. ESR1, ESR2 and FSH receptor gene polymorphisms in combination: a useful genetic tool for the prediction of poor responders. Curr Pharm Biotechnol. 2012, 13:426-34. 10.2174\/138920112799361891","journal-title":"Curr Pharm Biotechnol"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-014-0403-0","article-title":"Single nucleotide polymorphisms in the Anti-M\u00fcllerian hormone (AMH Ile(49)Ser) and Anti-M\u00fcllerian hormone type II receptor (AMHRII -482 A>G) as genetic markers in assisted reproduction technology","volume":"32","author":"Karagiorga I","year":"2015","unstructured":"Karagiorga I, Partsinevelos GA, Mavrogianni D, et al.. Single nucleotide polymorphisms in the Anti-M\u00fcllerian hormone (AMH Ile(49)Ser) and Anti-M\u00fcllerian hormone type II receptor (AMHRII -482 A>G) as genetic markers in assisted reproduction technology. J Assist Reprod Genet. 2015, 32:357-67. 10.1007\/s10815-014-0403-0","journal-title":"J Assist Reprod Genet"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deu025","article-title":"IVF culture medium affects post-natal weight in humans during the first 2 years of life","volume":"29","author":"Kleijkers SH","year":"2014","unstructured":"Kleijkers SH, van Montfoort AP, Smits LJ, et al.. IVF culture medium affects post-natal weight in humans during the first 2 years of life. Hum Reprod. 2014, 29:661-9. 10.1093\/humrep\/deu025","journal-title":"Hum Reprod"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016\/s1472-6483(10)61185-6","article-title":"Effects of culture medium on HCG concentrations and their value in predicting successful IVF outcome","volume":"12","author":"Orasanu B","year":"2006","unstructured":"Orasanu B, Jackson KV, Hornstein MD, Racowsky C. Effects of culture medium on HCG concentrations and their value in predicting successful IVF outcome. Reprod Biomed Online. 2006, 12:590-8. 10.1016\/s1472-6483(10)61185-6","journal-title":"Reprod Biomed Online"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dex366","article-title":"The epigenetic control of transposable elements and imprinted genes in newborns is affected by the mode of conception: ART versus spontaneous conception without underlying infertility","volume":"33","author":"Choux C","year":"2018","unstructured":"Choux C, Binquet C, Carmignac V, et al.. The epigenetic control of transposable elements and imprinted genes in newborns is affected by the mode of conception: ART versus spontaneous conception without underlying infertility. Hum Reprod. 2018, 33:331-40. 10.1093\/humrep\/dex366","journal-title":"Hum Reprod"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dei165","article-title":"Cell-free fetal DNA levels in pregnancies conceived by IVF","volume":"20","author":"Pan PD","year":"2005","unstructured":"Pan PD, Peter I, Lambert-Messerlian GM, Canick JA, Bianchi DW, Johnson KL. Cell-free fetal DNA levels in pregnancies conceived by IVF. Hum Reprod. 2005, 20:3152-6. 10.1093\/humrep\/dei165","journal-title":"Hum Reprod"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1038\/gim.2013.149","article-title":"Maternal plasma DNA testing for aneuploidy in pregnancies achieved by assisted reproductive technologies","volume":"16","author":"Lambert-Messerlian G","year":"2014","unstructured":"Lambert-Messerlian G, Kloza EM, Williams J 3rd, et al.. Maternal plasma DNA testing for aneuploidy in pregnancies achieved by assisted reproductive technologies. Genet Med. 2014, 16:419-22. 10.1038\/gim.2013.149","journal-title":"Genet Med"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1038\/gim.2018.4","article-title":"Cell-free fetal DNA versus maternal serum screening for trisomy 21 in pregnant women with and without assisted reproduction technology: a prospective interventional study","volume":"20","author":"Costa JM","year":"2018","unstructured":"Costa JM, Letourneau A, Favre R, et al.. Cell-free fetal DNA versus maternal serum screening for trisomy 21 in pregnant women with and without assisted reproduction technology: a prospective interventional study. Genet Med. 2018, 20:1346-53. 10.1038\/gim.2018.4","journal-title":"Genet Med"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deaa110","article-title":"Fetal fraction of cell-free DNA in pregnancies after fresh or frozen embryo transfer following assisted reproductive technologies","volume":"35","author":"Talbot AL","year":"2020","unstructured":"Talbot AL, Ambye L, Hartwig TS, et al.. Fetal fraction of cell-free DNA in pregnancies after fresh or frozen embryo transfer following assisted reproductive technologies. Hum Reprod. 2020, 35:1267-75. 10.1093\/humrep\/deaa110","journal-title":"Hum Reprod"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1016\/s0015-0282(99)00233-2","article-title":"Oocyte morphology correlates with embryo quality and pregnancy rate after intracytoplasmic sperm injection","volume":"72","author":"Loutradis D","year":"1999","unstructured":"Loutradis D, Drakakis P, Kallianidis K, Milingos S, Dendrinos S, Michalas S. Oocyte morphology correlates with embryo quality and pregnancy rate after intracytoplasmic sperm injection. Fertil Steril. 1999, 72:240-4. 10.1016\/s0015-0282(99)00233-2","journal-title":"Fertil Steril"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2000.tb06581.x","article-title":"An overview on the isolation and analysis of circulating tumor DNA in plasma and serum","volume":"906","author":"Jen J","year":"2000","unstructured":"Jen J, Wu L, Sidransky D. An overview on the isolation and analysis of circulating tumor DNA in plasma and serum. Ann N Y Acad Sci. 2000, 906:8-12. 10.1111\/j.1749-6632.2000.tb06581.x","journal-title":"Ann N Y Acad Sci"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1007\/978-90-481-9382-0_21","article-title":"The correlation of circulating cell-free DNA, cell-free fetal DNA and microRNA 325 levels to clinical characteristics and laboratory parameters in pre-eclampsia","author":"L\u00e1z\u00e1r L","year":"2011","unstructured":"L\u00e1z\u00e1r L, Nagy B, Morvarec A, Rig\u00f3 J. The correlation of circulating cell-free DNA, cell-free fetal DNA and microRNA 325 levels to clinical characteristics and laboratory parameters in pre-eclampsia. Circulating Nucleic Acids in Plasma and Serum. Gahan P (ed): Springer, Dordrecht; 2011. 153-6. 10.1007\/978-90-481-9382-0_21"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1530\/REP-09-0187","article-title":"The impact of ovarian stimulation for IVF on the developing embryo","volume":"139","author":"Santos MA","year":"2010","unstructured":"Santos MA, Kuijk EW, Macklon NS. The impact of ovarian stimulation for IVF on the developing embryo. Reproduction. 2010, 139:23-34. 10.1530\/REP-09-0187","journal-title":"Reproduction"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.15663","article-title":"Past, present, and future of gonadotropin use in controlled ovarian stimulation during assisted reproductive techniques","volume":"13","author":"Prodromidou A","year":"2021","unstructured":"Prodromidou A, Anagnostou E, Mavrogianni D, Liokari E, Dimitroulia E, Drakakis P, Loutradis D. Past, present, and future of gonadotropin use in controlled ovarian stimulation during assisted reproductive techniques. Cureus. 2021, 13:e15663. 10.7759\/cureus.15663","journal-title":"Cureus"}],"container-title":["Cureus"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cureus.com\/articles\/87765-comparison-of-the-fetal-fraction-of-cell-free-dna-in-in-vitro-fertilization-ivf-versus-natural-conception-evaluation-of-the-fetal-fraction-with-ivf-parameters","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,9]],"date-time":"2024-02-09T19:38:57Z","timestamp":1707507537000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cureus.com\/articles\/87765-comparison-of-the-fetal-fraction-of-cell-free-dna-in-in-vitro-fertilization-ivf-versus-natural-conception-evaluation-of-the-fetal-fraction-with-ivf-parameters"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,26]]},"references-count":29,"URL":"https:\/\/doi.org\/10.7759\/cureus.24516","relation":{},"ISSN":["2168-8184"],"issn-type":[{"value":"2168-8184","type":"print"}],"subject":[],"published":{"date-parts":[[2022,4,26]]}}}