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October 12, 2024: https:\/\/stacks.cdc.gov\/view\/cdc\/85935."},{"key":"ref83","doi-asserted-by":"publisher","DOI":"10.3121\/cmr.2012.1084","article-title":"Comparison of nasal and nasopharyngeal swabs for influenza detection in adults","volume":"10","author":"Irving SA","year":"2012","unstructured":"Irving SA, Vandermause MF, Shay DK, Belongia EA. Comparison of nasal and nasopharyngeal swabs for influenza detection in adults. Clin Med Res. 2012, 10:215-8. 10.3121\/cmr.2012.1084","journal-title":"Clin Med Res"},{"key":"ref84","doi-asserted-by":"publisher","DOI":"10.1016\/j.matt.2020.09.021","article-title":"Reducing false negatives in COVID-19 testing by using microneedle-based oropharyngeal swabs","volume":"3","author":"Chen W","year":"2020","unstructured":"Chen W, Cai B, Geng Z, Chen F, Wang Z, Wang L, Chen X. Reducing false negatives in COVID-19 testing by using microneedle-based oropharyngeal swabs. 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Ann Clin Lab Sci. 2004, 34:389-96.","journal-title":"Ann Clin Lab Sci"},{"key":"ref101","doi-asserted-by":"publisher","DOI":"10.1016\/j.mehy.2020.109781","article-title":"Airborne route and bad use of ventilation systems as non-negligible factors in SARS-CoV-2 transmission","volume":"141","author":"Correia G","year":"2020","unstructured":"Correia G, Rodrigues L, Gameiro da Silva M, Gon\u00e7alves T. Airborne route and bad use of ventilation systems as non-negligible factors in SARS-CoV-2 transmission. Med Hypotheses. 2020, 141:109781. 10.1016\/j.mehy.2020.109781","journal-title":"Med Hypotheses"},{"key":"ref102","doi-asserted-by":"publisher","DOI":"10.3402\/ehtj.v8.24173","article-title":"Hospital preparedness in community measles outbreaks-challenges and recommendations for low-resource settings","volume":"8","author":"Shakoor S","year":"2015","unstructured":"Shakoor S, Mir F, Zaidi AK, Zafar A. Hospital preparedness in community measles outbreaks-challenges and recommendations for low-resource settings. Emerg Health Threats J. 2015, 8:24173. 10.3402\/ehtj.v8.24173","journal-title":"Emerg Health Threats J"},{"key":"ref103","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000563","article-title":"Monogenic, polygenic, and oligogenic familial hypercholesterolemia","volume":"30","author":"Tada H","year":"2019","unstructured":"Tada H, Nohara A, Kawashiri MA. Monogenic, polygenic, and oligogenic familial hypercholesterolemia. Curr Opin Lipidol. 2019, 30:300-6. 10.1097\/QCO.0000000000000563","journal-title":"Curr Opin Lipidol"},{"key":"ref104","doi-asserted-by":"publisher","DOI":"10.1177\/1750458920949453","article-title":"Negative pressure rooms and COVID-19","volume":"31","author":"Al-Benna S","year":"2021","unstructured":"Al-Benna S. Negative pressure rooms and COVID-19. J Perioper Pract. 2021, 31:18-23. 10.1177\/1750458920949453","journal-title":"J Perioper Pract"},{"key":"ref105","doi-asserted-by":"publisher","DOI":"10.1016\/S0195-6701(07)60014-0","article-title":"Hospital and community acquired infection and the built environment--design and testing of infection control rooms","volume":"65 Suppl 2","author":"Walker JT","year":"2007","unstructured":"Walker JT, Hoffman P, Bennett AM, Vos MC, Thomas M, Tomlinson N. Hospital and community acquired infection and the built environment--design and testing of infection control rooms. J Hosp Infect. 2007, 65 Suppl 2:43-9. 10.1016\/S0195-6701(07)60014-0","journal-title":"J Hosp Infect"},{"key":"ref106","doi-asserted-by":"publisher","DOI":"10.1038\/339237a0","article-title":"Avoiding false positives with PCR","volume":"339","author":"Kwok S","year":"1989","unstructured":"Kwok S, Higuchi R. Avoiding false positives with PCR. Nature. 1989, 339:237-8. 10.1038\/339237a0","journal-title":"Nature"},{"key":"ref107","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2401579","article-title":"UV and clean air result in contamination-free PCR","volume":"13","author":"Padua RA","year":"1999","unstructured":"Padua RA, Parrado A, Larghero J, Chomienne C. UV and clean air result in contamination-free PCR. Leukemia. 1999, 13:1898-9. 10.1038\/sj.leu.2401579","journal-title":"Leukemia"},{"key":"ref108","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(20)30121-X","article-title":"Assessing a novel, lab-free, point-of-care test for SARS-CoV-2 (CovidNudge): a diagnostic accuracy study","volume":"1","author":"Gibani MM","year":"2020","unstructured":"Gibani MM, Toumazou C, Sohbati M, et al.. Assessing a novel, lab-free, point-of-care test for SARS-CoV-2 (CovidNudge): a diagnostic accuracy study. Lancet Microbe. 2020, 1:e300-7. 10.1016\/S2666-5247(20)30121-X","journal-title":"Lancet Microbe"},{"key":"ref109","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)92180-p","article-title":"Polymerase chain reaction decontamination: the wipe test. Polymerase chain reaction decontamination: the wipe test","volume":"336","author":"Cone R","year":"1990","unstructured":"Cone R, Hobson A, Huang M, Fairfax M. Polymerase chain reaction decontamination: the wipe test. Polymerase chain reaction decontamination: the wipe test. Lancet Microbe. 1990, 336:686-7. 10.1016\/0140-6736(90)92180-p","journal-title":"Lancet Microbe"},{"key":"ref110","doi-asserted-by":"publisher","DOI":"10.1016\/s0198-8859(97)00011-6","article-title":"Quality control for DNA contamination in laboratories using PCR-based class II HLA typing methods","volume":"54","author":"McCormack JM","year":"1997","unstructured":"McCormack JM, Sherman ML, Maurer DH. Quality control for DNA contamination in laboratories using PCR-based class II HLA typing methods. Hum Immunol. 1997, 54:82-8. 10.1016\/s0198-8859(97)00011-6","journal-title":"Hum Immunol"},{"key":"ref111","article-title":"Parameters affecting susceptibility of PCR contamination to UV inactivation","volume":"10","author":"Sarkar G","year":"1991","unstructured":"Sarkar G, Sommer SS. Parameters affecting susceptibility of PCR contamination to UV inactivation. Biotechniques. 1991, 10:590-4.","journal-title":"Biotechniques"},{"key":"ref112","article-title":"Use of UV irradiation to reduce false positivity in polymerase chain reaction","volume":"10","author":"Ou CY","year":"1991","unstructured":"Ou CY, Moore JL, Schochetman G. Use of UV irradiation to reduce false positivity in polymerase chain reaction. Biotechniques. 1991, 10:442, 444, 446.","journal-title":"Biotechniques"},{"key":"ref113","article-title":"Introduction to the 13th Edition of the Manual of Clinical Microbiology","author":"Carroll KC","year":"2023","unstructured":"Carroll KC, Pfaller MA. Introduction to the 13th Edition of the Manual of Clinical Microbiology. Manual of Clinical Microbiology. Wiley (Online), New Jersey; 2023."},{"key":"ref114","doi-asserted-by":"publisher","DOI":"10.1006\/mcpr.1993.1031","article-title":"Inhibition of in vitro enzymatic DNA amplification reaction by ultra-violet light irradiation","volume":"7","author":"Pao CC","year":"1993","unstructured":"Pao CC, Hor JJ, Tsai PL, Horng MY. Inhibition of in vitro enzymatic DNA amplification reaction by ultra-violet light irradiation. Mol Cell Probes. 1993, 7:217-9. 10.1006\/mcpr.1993.1031","journal-title":"Mol Cell Probes"},{"key":"ref115","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2020.110092","article-title":"Decay of SARS-CoV-2 and surrogate murine hepatitis virus RNA in untreated wastewater to inform application in wastewater-based epidemiology","volume":"191","author":"Ahmed W","year":"2020","unstructured":"Ahmed W, Bertsch PM, Bibby K, et al.. Decay of SARS-CoV-2 and surrogate murine hepatitis virus RNA in untreated wastewater to inform application in wastewater-based epidemiology. Environ Res. 2020, 191:110092. 10.1016\/j.envres.2020.110092","journal-title":"Environ Res"},{"key":"ref116","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.3.830-831.1999","article-title":"Identification of contaminating fungal DNA sequences in Zymolyase","volume":"37","author":"Rimek D","year":"1999","unstructured":"Rimek D, Garg AP, Haas WH, Kappe R. Identification of contaminating fungal DNA sequences in Zymolyase. J Clin Microbiol. 1999, 37:830-1. 10.1128\/JCM.37.3.830-831.1999","journal-title":"J Clin Microbiol"},{"key":"ref117","doi-asserted-by":"crossref","DOI":"10.1093\/clinchem\/36.6.1258b","article-title":"Frequent contamination of Taq polymerase with DNA","volume":"36","author":"B\u00f6ttger EC","year":"1990","unstructured":"B\u00f6ttger EC. Frequent contamination of Taq polymerase with DNA. Clin Chem. 1990, 36:1258-9.","journal-title":"Clin Chem"},{"key":"ref118","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/hvaa214","article-title":"Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2","volume":"66","author":"Huggett JF","year":"2020","unstructured":"Huggett JF, Benes V, Bustin SA, et al.. Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2. Clin Chem. 2020, 66:1369-72. 10.1093\/clinchem\/hvaa214","journal-title":"Clin Chem"},{"key":"ref119","doi-asserted-by":"publisher","DOI":"10.1007\/BF01133677","article-title":"Evidence of contamination in PCR laboratory disposables","volume":"82","author":"Schmidt T","year":"1995","unstructured":"Schmidt T, Hummel S, Herrmann B. Evidence of contamination in PCR laboratory disposables. Naturwissenschaften. 1995, 82:423-31. 10.1007\/BF01133677","journal-title":"Naturwissenschaften"},{"key":"ref120","article-title":"Laboratory experience and guidelines for avoiding false positive polymerase chain reaction results","volume":"31","author":"Victor T","year":"1993","unstructured":"Victor T, Jordaan A, du Toit R, Van Helden PD. Laboratory experience and guidelines for avoiding false positive polymerase chain reaction results. Eur J Clin Chem Clin Biochem. 1993, 31:531-5.","journal-title":"Eur J Clin Chem Clin Biochem"},{"key":"ref121","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.19.2189","article-title":"Reaction of sodium hypochlorite with nucleic acids and their constituents","volume":"19","author":"Hayatsu H","year":"1971","unstructured":"Hayatsu H, Pan S, Ukita T. Reaction of sodium hypochlorite with nucleic acids and their constituents. Chem Pharm Bull (Tokyo). 1971, 19:2189-92. 10.1248\/cpb.19.2189","journal-title":"Chem Pharm Bull (Tokyo)"},{"key":"ref122","doi-asserted-by":"publisher","DOI":"10.1016\/j.amsu.2021.102320","article-title":"Positive COVID19-PCR patients as negative controls for COVID19 antibody tests","volume":"65","author":"Alrabadi N","year":"2021","unstructured":"Alrabadi N, Haddad R, Al-Faouri I, et al.. Positive COVID19-PCR patients as negative controls for COVID19 antibody tests. 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