{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T10:16:12Z","timestamp":1769163372366,"version":"3.49.0"},"reference-count":25,"publisher":"Springer Science and Business Media LLC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,15]]},"DOI":"10.7759\/cureus.77495","type":"journal-article","created":{"date-parts":[[2025,1,15]],"date-time":"2025-01-15T20:04:18Z","timestamp":1736971458000},"source":"Crossref","is-referenced-by-count":1,"title":["Management of Complex Perianal Fistulas Using Platelet-Rich Plasma and Adipose-Derived Mesenchymal Stem Cells: A Case Series"],"prefix":"10.7759","author":[{"given":"Patroklos","family":"Goulas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Karakwta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra-Eleftheria","family":"Menni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Apostolos","family":"Zatagias","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandros","family":"Zevgaridis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia Valeria","family":"Pentara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aristeidis","family":"Ioannidis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stavros","family":"Panidis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Despoina","family":"Krokou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikolaos","family":"Gkouliaveras","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stylianos","family":"Apostolidis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonios","family":"Michalopoulos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Koliakos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vasileios","family":"Papadopoulos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","reference":[{"key":"ref1","doi-asserted-by":"crossref","DOI":"10.1002\/tre.704","article-title":"Anal fistulae: an ancient problem; a challenge today","author":"Soliman F","year":"2019","unstructured":"Soliman F, Hargest R. Anal fistulae: an ancient problem; a challenge today. Trends Urol Men\u2019s Health. 2019,","journal-title":"Trends Urol Men\u2019s Health"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v17.i28.3277","article-title":"Idiopathic fistula-in-ano","volume":"17","author":"Shawki S","year":"2011","unstructured":"Shawki S, Wexner SD. Idiopathic fistula-in-ano. World J Gastroenterol. 2011, 17:3277-85. 10.3748\/wjg.v17.i28.3277","journal-title":"World J Gastroenterol"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.surge.2016.02.002","article-title":"A systematic review of new treatments for cryptoglandular fistula in ano","volume":"15","author":"Narang SK","year":"2017","unstructured":"Narang SK, Keogh K, Alam NN, Pathak S, Daniels IR, Smart NJ. A systematic review of new treatments for cryptoglandular fistula in ano. Surgeon. 2017, 15:30-9. 10.1016\/j.surge.2016.02.002","journal-title":"Surgeon"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1097\/DCR.0000000000001797","article-title":"Modification of Parks classification of cryptoglandular anal fistula","volume":"64","author":"Emile SH","year":"2021","unstructured":"Emile SH, Elfeki H, El-Said M, Khafagy W, Shalaby M. Modification of Parks classification of cryptoglandular anal fistula. Dis Colon Rectum. 2021, 64:446-58. 10.1097\/DCR.0000000000001797","journal-title":"Dis Colon Rectum"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijsu.2017.04.019","article-title":"Comparing existing classifications of fistula-in-ano in 440 operated patients: is it time for a new classification? A Retrospective Cohort Study","volume":"42","author":"Garg P","year":"2017","unstructured":"Garg P. Comparing existing classifications of fistula-in-ano in 440 operated patients: is it time for a new classification? A Retrospective Cohort Study. Int J Surg. 2017, 42:34-40. 10.1016\/j.ijsu.2017.04.019","journal-title":"Int J Surg"},{"key":"ref6","article-title":"Precise and comprehensive evaluation of perianal fistulas, classification and related complications using magnetic resonance imaging","volume":"15","author":"Zhao WW","year":"2023","unstructured":"Zhao WW, Yu J, Shu J, et al.. Precise and comprehensive evaluation of perianal fistulas, classification and related complications using magnetic resonance imaging. Am J Transl Res. 2023, 15:3674-85.","journal-title":"Am J Transl Res"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.1800630102","article-title":"A classification of fistula-in-ano","volume":"63","author":"Parks AG","year":"1976","unstructured":"Parks AG, Gordon PH, Hardcastle JD. A classification of fistula-in-ano. Br J Surg. 1976, 63:1-12. 10.1002\/bjs.1800630102","journal-title":"Br J Surg"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.57137","article-title":"A simple and effective mechanical method for adipose-derived stromal vascular fraction isolation","volume":"16","author":"Goulas P","year":"2024","unstructured":"Goulas P, Karakwta M, Zatagias A, et al.. A simple and effective mechanical method for adipose-derived stromal vascular fraction isolation. Cureus. 2024, 16:e57137. 10.7759\/cureus.57137","journal-title":"Cureus"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.21026","article-title":"Etiology of perianal Crohn's disease: role of genetic, microbiological, and immunological factors","volume":"15","author":"Tozer PJ","year":"2009","unstructured":"Tozer PJ, Whelan K, Phillips RK, Hart AL. Etiology of perianal Crohn's disease: role of genetic, microbiological, and immunological factors. Inflamm Bowel Dis. 2009, 15:1591-8. 10.1002\/ibd.21026","journal-title":"Inflamm Bowel Dis"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcot.2021.101534","article-title":"Human platelet lysate - a potent (and overlooked) orthobiologic","volume":"21","author":"da Fonseca L","year":"2021","unstructured":"da Fonseca L, Santos GS, Huber SC, Setti TM, Setti T, Lana JF. Human platelet lysate - a potent (and overlooked) orthobiologic. J Clin Orthop Trauma. 2021, 21:101534. 10.1016\/j.jcot.2021.101534","journal-title":"J Clin Orthop Trauma"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviscsurg.2010.07.007","article-title":"Cryptoglandular anal fistula","volume":"147","author":"de Parades V","year":"2010","unstructured":"de Parades V, Zeitoun JD, Atienza P. Cryptoglandular anal fistula. J Visc Surg. 2010, 147:e203-15. 10.1016\/j.jviscsurg.2010.07.007","journal-title":"J Visc Surg"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2017.104","article-title":"Perianal fistulizing Crohn's disease: pathogenesis, diagnosis and therapy","volume":"14","author":"Pan\u00e9s J","year":"2017","unstructured":"Pan\u00e9s J, Rimola J. Perianal fistulizing Crohn's disease: pathogenesis, diagnosis and therapy. Nat Rev Gastroenterol Hepatol. 2017, 14:652-64. 10.1038\/nrgastro.2017.104","journal-title":"Nat Rev Gastroenterol Hepatol"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1111\/codi.12810","article-title":"What role do bacteria play in persisting fistula formation in idiopathic and Crohn's anal fistula?","volume":"17","author":"Tozer PJ","year":"2015","unstructured":"Tozer PJ, Rayment N, Hart AL, Daulatzai N, Murugananthan AU, Whelan K, Phillips RK. What role do bacteria play in persisting fistula formation in idiopathic and Crohn's anal fistula?. Colorectal Dis. 2015, 17:235-41. 10.1111\/codi.12810","journal-title":"Colorectal Dis"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1111\/codi.16290","article-title":"The pathogenesis of cryptoglandular anal fistula: new insight into the immunological profile","volume":"24","author":"Litta F","year":"2022","unstructured":"Litta F, Papait A, Lucchetti D, et al.. The pathogenesis of cryptoglandular anal fistula: new insight into the immunological profile. Colorectal Dis. 2022, 24:1567-75. 10.1111\/codi.16290","journal-title":"Colorectal Dis"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-012-8347-6","article-title":"Use of mesenchymal stem cells (MSC) in chronic inflammatory fistulizing and fibrotic diseases: a comprehensive review","volume":"45","author":"Voswinkel J","year":"2013","unstructured":"Voswinkel J, Francois S, Simon JM, et al.. Use of mesenchymal stem cells (MSC) in chronic inflammatory fistulizing and fibrotic diseases: a comprehensive review. Clin Rev Allergy Immunol. 2013, 45:180-92. 10.1007\/s12016-012-8347-6","journal-title":"Clin Rev Allergy Immunol"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1093\/ecco-jcc\/jjx046","article-title":"Mesenchymal stem cell injections for the treatment of perianal Crohn\u2019s disease: what we have accomplished and what we still need to do","volume":"11","author":"Lightner AL","year":"2017","unstructured":"Lightner AL, Faubion WA. Mesenchymal stem cell injections for the treatment of perianal Crohn\u2019s disease: what we have accomplished and what we still need to do. J Crohns Colitis. 2017, 11:1267-76. 10.1093\/ecco-jcc\/jjx046","journal-title":"J Crohns Colitis"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-017-0598-y","article-title":"Adipose tissue-derived stromal vascular fraction in regenerative medicine: a brief review on biology and translation","volume":"8","author":"Bora P","year":"2017","unstructured":"Bora P, Majumdar AS. Adipose tissue-derived stromal vascular fraction in regenerative medicine: a brief review on biology and translation. Stem Cell Res Ther. 2017, 8:145. 10.1186\/s13287-017-0598-y","journal-title":"Stem Cell Res Ther"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.17235\/reed.2016.3946\/2015","article-title":"Using autologous platelet-rich plasma for the treatment of complex fistulas","volume":"108","author":"Moreno-Serrano A","year":"2016","unstructured":"Moreno-Serrano A, Garc\u00eda-D\u00edaz JJ, Ferrer-M\u00e1rquez M, Alarc\u00f3n-Rodr\u00edguez R, \u00c1lvarez-Garc\u00eda A, Reina-Duarte \u00c1. Using autologous platelet-rich plasma for the treatment of complex fistulas. Rev Esp Enferm Dig. 2016, 108:123-8. 10.17235\/reed.2016.3946\/2015","journal-title":"Rev Esp Enferm Dig"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.3760\/cma.j.issn.1009-2587.2017.01.004","article-title":"Experimental research on the effects of different activators on the formation of platelet-rich gel and the release of bioactive substances in human platelet-rich plasma [Article in Chinese]","volume":"33","author":"Yang Y","year":"2017","unstructured":"Yang Y, Zhang W, Cheng B. Experimental research on the effects of different activators on the formation of platelet-rich gel and the release of bioactive substances in human platelet-rich plasma [Article in Chinese]. Zhonghua Shao Shang Za Zhi. 2017, 33:12-17. 10.3760\/cma.j.issn.1009-2587.2017.01.004","journal-title":"Zhonghua Shao Shang Za Zhi"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1097\/DCR.0000000000000023","article-title":"Long-term results of mucosal advancement flap combined with platelet-rich plasma for high cryptoglandular perianal fistulas","volume":"57","author":"G\u00f6ttgens KW","year":"2014","unstructured":"G\u00f6ttgens KW, Vening W, van der Hagen SJ, et al.. Long-term results of mucosal advancement flap combined with platelet-rich plasma for high cryptoglandular perianal fistulas. Dis Colon Rectum. 2014, 57:223-7. 10.1097\/DCR.0000000000000023","journal-title":"Dis Colon Rectum"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2023.103039","article-title":"Platelet-Rich stroma from Crohn's disease patients for treatment of perianal fistula shows a higher myeloid cell profile compared to non-IBD controls","volume":"67","author":"Arkenbosch JH","year":"2023","unstructured":"Arkenbosch JH, Becker MA, Buskens CJ, et al.. Platelet-Rich stroma from Crohn's disease patients for treatment of perianal fistula shows a higher myeloid cell profile compared to non-IBD controls. Stem Cell Res. 2023, 67:103039. 10.1016\/j.scr.2023.103039","journal-title":"Stem Cell Res"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1097\/DCR.0000000000001924","article-title":"Efficacy and safety of treatment of complex idiopathic fistula-in-ano using autologous centrifuged adipose tissue containing progenitor cells: a randomized controlled trial","volume":"64","author":"Ascanelli S","year":"2021","unstructured":"Ascanelli S, Zamboni P, Campioni D, et al.. Efficacy and safety of treatment of complex idiopathic fistula-in-ano using autologous centrifuged adipose tissue containing progenitor cells: a randomized controlled trial. Dis Colon Rectum. 2021, 64:1276-85. 10.1097\/DCR.0000000000001924","journal-title":"Dis Colon Rectum"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1097\/DCR.0000000000002379","article-title":"Efficacy of injection of freshly collected autologous adipose tissue into complex cryptoglandular anal fistulas","volume":"66","author":"Dalby HR","year":"2023","unstructured":"Dalby HR, Dige A, Pedersen BG, Krogh K, Agnholt J, Hougaard HT, Lundby L. Efficacy of injection of freshly collected autologous adipose tissue into complex cryptoglandular anal fistulas. Dis Colon Rectum. 2023, 66:443-50. 10.1097\/DCR.0000000000002379","journal-title":"Dis Colon Rectum"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1111\/apt.17347","article-title":"Stromal vascular fraction with platelet-rich plasma injection during surgery is feasible and safe in treatment-refractory perianal fistulising Crohn's disease: a pilot study","volume":"57","author":"Arkenbosch JH","year":"2023","unstructured":"Arkenbosch JH, van Ruler O, Dwarkasing RS, et al.. Stromal vascular fraction with platelet-rich plasma injection during surgery is feasible and safe in treatment-refractory perianal fistulising Crohn's disease: a pilot study. Aliment Pharmacol Ther. 2023, 57:783-91. 10.1111\/apt.17347","journal-title":"Aliment Pharmacol Ther"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines11092509","article-title":"Novel approach in rectovaginal fistula treatment: combination of modified Martius flap and autologous micro-fragmented adipose tissue","volume":"11","author":"Dimova A","year":"2023","unstructured":"Dimova A, Erceg Ivko\u0161i\u0107 I, Brlek P, Dimov S, Pavlovi\u0107 T, Bokun T, Primorac D. Novel approach in rectovaginal fistula treatment: combination of modified Martius flap and autologous micro-fragmented adipose tissue. Biomedicines. 2023, 11:2509. 10.3390\/biomedicines11092509","journal-title":"Biomedicines"}],"container-title":["Cureus"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cureus.com\/articles\/320848-management-of-complex-perianal-fistulas-using-platelet-rich-plasma-and-adipose-derived-mesenchymal-stem-cells-a-case-series","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,15]],"date-time":"2025-01-15T20:04:24Z","timestamp":1736971464000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cureus.com\/articles\/320848-management-of-complex-perianal-fistulas-using-platelet-rich-plasma-and-adipose-derived-mesenchymal-stem-cells-a-case-series"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,15]]},"references-count":25,"URL":"https:\/\/doi.org\/10.7759\/cureus.77495","relation":{},"ISSN":["2168-8184"],"issn-type":[{"value":"2168-8184","type":"print"}],"subject":[],"published":{"date-parts":[[2025,1,15]]}}}