{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,15]],"date-time":"2025-08-15T01:29:19Z","timestamp":1755221359982,"version":"3.43.0"},"reference-count":8,"publisher":"Springer Science and Business Media LLC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,8,8]]},"DOI":"10.7759\/cureus.89628","type":"journal-article","created":{"date-parts":[[2025,8,8]],"date-time":"2025-08-08T16:43:58Z","timestamp":1754671438000},"source":"Crossref","is-referenced-by-count":0,"title":["Optimization of Culture Conditions for Human Ovarian Stem Cells Using Gelatin-Coated Surfaces"],"prefix":"10.7759","author":[{"given":"Sindhuja N","family":"Srinivasan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanjeeva","family":"Reddy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan M","family":"Punnoose","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mubeena","family":"S","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0031-1280921","article-title":"Life plans and family-building options for women with primary ovarian insufficiency","volume":"29","author":"Baker V","year":"2011","unstructured":"Baker V. Life plans and family-building options for women with primary ovarian insufficiency. Semin Reprod Med. 2011, 29:362-72. 10.1055\/s-0031-1280921","journal-title":"Semin Reprod Med"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.06.031","article-title":"Oocyte generation in adult mammalian ovaries by putative germ cells in bone marrow and peripheral blood","volume":"122","author":"Johnson J","year":"2005","unstructured":"Johnson J, Bagley J, Skaznik-Wikiel M, et al.. Oocyte generation in adult mammalian ovaries by putative germ cells in bone marrow and peripheral blood. Cell. 2005, 122:303-15. 10.1016\/j.cell.2005.06.031","journal-title":"Cell"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2008-0233","article-title":"The multipotency of luteinizing granulosa cells collected from mature ovarian follicles","volume":"27","author":"Kossowska-Tomaszczuk K","year":"2009","unstructured":"Kossowska-Tomaszczuk K, De Geyter C, De Geyter M, Martin I, Holzgreve W, Scherberich A, Zhang H. The multipotency of luteinizing granulosa cells collected from mature ovarian follicles. Stem Cells. 2009, 27:210-9. 10.1634\/stemcells.2008-0233","journal-title":"Stem Cells"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.22-5-790","article-title":"Derivation of human embryonic stem cells from day-8 blastocysts recovered after three-step in vitro culture","volume":"22","author":"Stojkovic M","year":"2004","unstructured":"Stojkovic M, Lako M, Stojkovic P, et al.. Derivation of human embryonic stem cells from day-8 blastocysts recovered after three-step in vitro culture. Stem Cells. 2004, 22:790-7. 10.1634\/stemcells.22-5-790","journal-title":"Stem Cells"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1038\/196476a0","article-title":"Effect of absorbed proteins on the wettability of hydrophilic and hydrophobic solids","volume":"196","author":"VR L","year":"1962","unstructured":"VR L. Effect of absorbed proteins on the wettability of hydrophilic and hydrophobic solids. Nature. 1962, 196:476-7. 10.1038\/196476a0","journal-title":"Nature"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1631","article-title":"Synthetic polymer coatings for long-term growth of human embryonic stem cells","volume":"28","author":"Villa-Diaz LG","year":"2010","unstructured":"Villa-Diaz LG, Nandivada H, Ding J, et al.. Synthetic polymer coatings for long-term growth of human embryonic stem cells. Nat Biotechnol. 2010, 28:581-3. 10.1038\/nbt.1631","journal-title":"Nat Biotechnol"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.08.007","article-title":"Long-term human pluripotent stem cell self-renewal on synthetic polymer surfaces","volume":"31","author":"Brafman DA","year":"2010","unstructured":"Brafman DA, Chang CW, Fernandez A, Willert K, Varghese S, Chien S. Long-term human pluripotent stem cell self-renewal on synthetic polymer surfaces. Biomaterials. 2010, 31:9135-44. 10.1016\/j.biomaterials.2010.08.007","journal-title":"Biomaterials"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3620","article-title":"Role of the extracellular matrix in regulating stem cell fate","volume":"14","author":"Watt FM","year":"2013","unstructured":"Watt FM, Huck WT. Role of the extracellular matrix in regulating stem cell fate. Nat Rev Mol Cell Biol. 2013, 14:467-73. 10.1038\/nrm3620","journal-title":"Nat Rev Mol Cell Biol"}],"container-title":["Cureus"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cureus.com\/articles\/76340-optimization-of-culture-conditions-for-human-ovarian-stem-cells-using-gelatin-coated-surfaces","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,8]],"date-time":"2025-08-08T16:43:58Z","timestamp":1754671438000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cureus.com\/articles\/76340-optimization-of-culture-conditions-for-human-ovarian-stem-cells-using-gelatin-coated-surfaces"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,8]]},"references-count":8,"URL":"https:\/\/doi.org\/10.7759\/cureus.89628","relation":{},"ISSN":["2168-8184"],"issn-type":[{"value":"2168-8184","type":"print"}],"subject":[],"published":{"date-parts":[[2025,8,8]]}}}