{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:32:13Z","timestamp":1761744733661,"version":"build-2065373602"},"reference-count":23,"publisher":"Springer Science and Business Media LLC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,10,29]]},"DOI":"10.7759\/cureus.95647","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T09:05:07Z","timestamp":1761728707000},"source":"Crossref","is-referenced-by-count":0,"title":["Histopathological Study of Regenerative Endodontic Therapy on an Immature Mandibular Second Premolar With Pulp Necrosis: A Case Report"],"prefix":"10.7759","author":[{"given":"Hidefumi","family":"Sako","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiro","family":"Omori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuki","family":"Shinoda-Ito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiyofumi","family":"Takabatake","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Nagatsuka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shogo","family":"Takashiba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.14219\/jada.archive.1966.0017","article-title":"Therapy for the divergent pulpless tooth by continued apical formation","volume":"72","author":"Frank AL","year":"1966","unstructured":"Frank AL. Therapy for the divergent pulpless tooth by continued apical formation. J Am Dent Assoc. 1966, 72:87-93. 10.14219\/jada.archive.1966.0017","journal-title":"J Am Dent Assoc"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cden.2016.08.009","article-title":"Endodontictreatment outcomes","volume":"61","author":"Chugal N","year":"2017","unstructured":"Chugal N, Mallya SM, Kahler B, Lin LM. Endodontictreatment outcomes. Dent Clin North Am. 2017, 61:59-80. 10.1016\/j.cden.2016.08.009","journal-title":"Dent Clin North Am"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-9657.2001.017004185.x","article-title":"Revascularization of an immature permanent tooth with apical periodontitis and sinus tract","volume":"17","author":"Iwaya SI","year":"2001","unstructured":"Iwaya SI, Ikawa M, Kubota M. Revascularization of an immature permanent tooth with apical periodontitis and sinus tract. Dent Traumatol. 2001, 17:185-7. 10.1034\/j.1600-9657.2001.017004185.x","journal-title":"Dent Traumatol"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1097\/00004770-200404000-00003","article-title":"Revascularization of immature permanent teeth with apical periodontitis: new treatment protocol?","volume":"30","author":"Banchs F","year":"2004","unstructured":"Banchs F, Trope M. Revascularization of immature permanent teeth with apical periodontitis: new treatment protocol?. J Endod. 2004, 30:196-200. 10.1097\/00004770-200404000-00003","journal-title":"J Endod"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2009.09.039","article-title":"Histologic characterization of regenerated tissues in canal space after the revitalization\/revascularization procedure of immature dog teeth with apical periodontitis","volume":"36","author":"Wang X","year":"2010","unstructured":"Wang X, Thibodeau B, Trope M, Lin LM, Huang GT. Histologic characterization of regenerated tissues in canal space after the revitalization\/revascularization procedure of immature dog teeth with apical periodontitis. J Endod. 2010, 36:56-63. 10.1016\/j.joen.2009.09.039","journal-title":"J Endod"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2011.05.014","article-title":"Response to intracanal medication in immature teeth with pulp necrosis: an experimental model in rat molars","volume":"37","author":"Scarparo RK","year":"2011","unstructured":"Scarparo RK, Dondoni L, B\u00f6ttcher DE, Grecca FS, Rockenbach MI, Batista EL Jr. Response to intracanal medication in immature teeth with pulp necrosis: an experimental model in rat molars. J Endod. 2011, 37:1069-73. 10.1016\/j.joen.2011.05.014","journal-title":"J Endod"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1111\/iej.12087","article-title":"Regeneration of dental pulp tissue in immature teeth with apical periodontitis using platelet-rich plasma and dental pulp cells","volume":"46","author":"Zhu W","year":"2013","unstructured":"Zhu W, Zhu X, Huang GT, Cheung GS, Dissanayaka WL, Zhang C. Regeneration of dental pulp tissue in immature teeth with apical periodontitis using platelet-rich plasma and dental pulp cells. Int Endod J. 2013, 46:962-70. 10.1111\/iej.12087","journal-title":"Int Endod J"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2015.03.012","article-title":"Histologic and immunohistochemical findings of a human immature permanent tooth with apical periodontitis after regenerative endodontic treatment","volume":"41","author":"Lei L","year":"2015","unstructured":"Lei L, Chen Y, Zhou R, Huang X, Cai Z. Histologic and immunohistochemical findings of a human immature permanent tooth with apical periodontitis after regenerative endodontic treatment. J Endod. 2015, 41:1172-9. 10.1016\/j.joen.2015.03.012","journal-title":"J Endod"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2012.03.006","article-title":"A clinical and histological report of a tooth with an open apex treated with regenerative endodontics using platelet-rich plasma","volume":"38","author":"Torabinejad M","year":"2012","unstructured":"Torabinejad M, Faras H. A clinical and histological report of a tooth with an open apex treated with regenerative endodontics using platelet-rich plasma. J Endod. 2012, 38:864-8. 10.1016\/j.joen.2012.03.006","journal-title":"J Endod"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2012.06.017","article-title":"Histologic observation of a human immature permanent tooth with irreversible pulpitis after revascularization\/regeneration procedure","volume":"38","author":"Shimizu E","year":"2012","unstructured":"Shimizu E, Jong G, Partridge N, Rosenberg PA, Lin LM. Histologic observation of a human immature permanent tooth with irreversible pulpitis after revascularization\/regeneration procedure. J Endod. 2012, 38:1293-7. 10.1016\/j.joen.2012.06.017","journal-title":"J Endod"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2012.09.015","article-title":"Histological findings of revascularized\/revitalized immature permanent molar with apical periodontitis using platelet-rich plasma","volume":"39","author":"Martin G","year":"2013","unstructured":"Martin G, Ricucci D, Gibbs JL, Lin LM. Histological findings of revascularized\/revitalized immature permanent molar with apical periodontitis using platelet-rich plasma. J Endod. 2013, 39:138-44. 10.1016\/j.joen.2012.09.015","journal-title":"J Endod"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2013.04.032","article-title":"Clinical, radiographic, and histological observation of a human immature permanent tooth with chronic apical abscess after revitalization treatment","volume":"39","author":"Shimizu E","year":"2013","unstructured":"Shimizu E, Ricucci D, Albert J, Alobaid AS, Gibbs JL, Huang GT, Lin LM. Clinical, radiographic, and histological observation of a human immature permanent tooth with chronic apical abscess after revitalization treatment. J Endod. 2013, 39:1078-83. 10.1016\/j.joen.2013.04.032","journal-title":"J Endod"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2013.07.017","article-title":"Histologic study of a human immature permanent premolar with chronic apical abscess after revascularization\/revitalization","volume":"40","author":"Becerra P","year":"2014","unstructured":"Becerra P, Ricucci D, Loghin S, Gibbs JL, Lin LM. Histologic study of a human immature permanent premolar with chronic apical abscess after revascularization\/revitalization. J Endod. 2014, 40:133-9. 10.1016\/j.joen.2013.07.017","journal-title":"J Endod"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2017.01.031","article-title":"Histologic findings of a human immature revascularized\/regenerated tooth with symptomatic irreversible pulpitis","volume":"43","author":"Peng C","year":"2017","unstructured":"Peng C, Zhao Y, Wang W, Yang Y, Qin M, Ge L. Histologic findings of a human immature revascularized\/regenerated tooth with symptomatic irreversible pulpitis. J Endod. 2017, 43:905-9. 10.1016\/j.joen.2017.01.031","journal-title":"J Endod"},{"key":"ref15","unstructured":"American Association of Endodontists. Scope of Endodontics, Regenerative Endodontics, AAE Position Statement. (2018). Accessed: September 25, 2025: http:\/\/https: \/\/www.aae.org\/specialty\/wp-content\/uploads\/sites\/2\/2018\/07\/2018AAE_Scope_of_Endo_Regenerative_Endodonti...."},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.1991.tb00117.x","article-title":"The antimicrobial effect of calcium hydroxide as a short-term intracanal dressing","volume":"24","author":"Sj\u00f6gren U","year":"1991","unstructured":"Sj\u00f6gren U, Figdor D, Sp\u00e5ngberg L, Sundqvist G. The antimicrobial effect of calcium hydroxide as a short-term intracanal dressing. Int Endod J. 1991, 24:119-25. 10.1111\/j.1365-2591.1991.tb00117.x","journal-title":"Int Endod J"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.2011.01978.x","article-title":"Responses of immature permanent teeth with infected necrotic pulp tissue and apical periodontitis\/abscess to revascularization procedures","volume":"45","author":"Chen MY","year":"2012","unstructured":"Chen MY, Chen KL, Chen CA, Tayebaty F, Rosenberg PA, Lin LM. Responses of immature permanent teeth with infected necrotic pulp tissue and apical periodontitis\/abscess to revascularization procedures. Int Endod J. 2012, 45:294-305. 10.1111\/j.1365-2591.2011.01978.x","journal-title":"Int Endod J"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.jobcr.2021.11.005","article-title":"Meta-analysis of regenerative endodontics outcomes with antibiotics pastes and calcium hydroxide. The apex of the iceberg","volume":"12","author":"B\u00e1ez V","year":"2022","unstructured":"B\u00e1ez V, Corcos L, Morgillo F, Imperatrice L, Gualtieri AF. Meta-analysis of regenerative endodontics outcomes with antibiotics pastes and calcium hydroxide. The apex of the iceberg. J Oral Biol Craniofac Res. 2022, 12:90-8. 10.1016\/j.jobcr.2021.11.005","journal-title":"J Oral Biol Craniofac Res"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1038\/s41368-022-00206-z","article-title":"Expert consensus on regenerative endodontic procedures","volume":"14","author":"Wei X","year":"2022","unstructured":"Wei X, Yang M, Yue L, et al.. Expert consensus on regenerative endodontic procedures. Int J Oral Sci. 2022, 14:55. 10.1038\/s41368-022-00206-z","journal-title":"Int J Oral Sci"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2019.02.002","article-title":"Evaluation of blood clot, platelet-rich plasma, platelet-rich fibrin, and platelet pellet as scaffolds in regenerative endodontic treatment: a prospective randomized trial","volume":"45","author":"Ulusoy AT","year":"2019","unstructured":"Ulusoy AT, Turedi I, Cimen M, Cehreli ZC. Evaluation of blood clot, platelet-rich plasma, platelet-rich fibrin, and platelet pellet as scaffolds in regenerative endodontic treatment: a prospective randomized trial. J Endod. 2019, 45:560-6. 10.1016\/j.joen.2019.02.002","journal-title":"J Endod"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1111\/edt.13002","article-title":"Transient apical breakdown: incidence, pathogenesis, and healing","volume":"41 Suppl 1","author":"Tsukiboshi M","year":"2025","unstructured":"Tsukiboshi M, Berlin-Broner Y, Levin L. Transient apical breakdown: incidence, pathogenesis, and healing. Dent Traumatol. 2025, 41 Suppl 1:72-9. 10.1111\/edt.13002","journal-title":"Dent Traumatol"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2023.05.017","article-title":"Histologic and electron microscopic characterization of a human immature permanent premolar with chronic apical abcess 16 years after regenerative endodontic procedures","volume":"49","author":"Kwon SK","year":"2023","unstructured":"Kwon SK, Kyeong M, Adasooriya D, Cho SW, Jung IY. Histologic and electron microscopic characterization of a human immature permanent premolar with chronic apical abcess 16 years after regenerative endodontic procedures. J Endod. 2023, 49:1051-7. 10.1016\/j.joen.2023.05.017","journal-title":"J Endod"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2020.01.015","article-title":"An immunofluorescence study to analyze wound healing outcomes of regenerative endodontics in an immature premolar with chronic apical abcess","volume":"46","author":"Lui JN","year":"2020","unstructured":"Lui JN, Lim WY, Ricucci D. An immunofluorescence study to analyze wound healing outcomes of regenerative endodontics in an immature premolar with chronic apical abcess. J Endod. 2020, 46:627-40. 10.1016\/j.joen.2020.01.015","journal-title":"J Endod"}],"container-title":["Cureus"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cureus.com\/articles\/424317-histopathological-study-of-regenerative-endodontic-therapy-on-an-immature-mandibular-second-premolar-with-pulp-necrosis-a-case-report","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T09:05:10Z","timestamp":1761728710000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cureus.com\/articles\/424317-histopathological-study-of-regenerative-endodontic-therapy-on-an-immature-mandibular-second-premolar-with-pulp-necrosis-a-case-report"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,29]]},"references-count":23,"URL":"https:\/\/doi.org\/10.7759\/cureus.95647","relation":{},"ISSN":["2168-8184"],"issn-type":[{"value":"2168-8184","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10,29]]}}}