{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T03:48:26Z","timestamp":1784864906751,"version":"3.55.0"},"reference-count":70,"publisher":"XMLink","issue":"1","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008257","name":"Tarbiat Modares University","doi-asserted-by":"publisher","award":["52D\/1649"],"award-info":[{"award-number":["52D\/1649"]}],"id":[{"id":"10.13039\/501100008257","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003968","name":"Iran National Science Foundation","doi-asserted-by":"publisher","award":["98008896"],"award-info":[{"award-number":["98008896"]}],"id":[{"id":"10.13039\/501100003968","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ecevr.org"],"crossmark-restriction":false},"short-container-title":["Clin Exp Vaccine Res"],"published-print":{"date-parts":[[2021]]},"DOI":"10.7774\/cevr.2021.10.1.59","type":"journal-article","created":{"date-parts":[[2021,2,18]],"date-time":"2021-02-18T01:20:41Z","timestamp":1613611241000},"page":"59","update-policy":"https:\/\/doi.org\/10.7774\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Immunoinformatic analysis of immunogenic B- and T-cell epitopes of MIC4 protein to designing a vaccine candidate against<i>Toxoplasma gondii<\/i>through an in-silico approach"],"prefix":"10.7774","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9635-2876","authenticated-orcid":false,"given":"Ali Dalir","family":"Ghaffari","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5591-5513","authenticated-orcid":false,"given":"Abdolhossein","family":"Dalimi","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0891-8214","authenticated-orcid":false,"given":"Fatemeh","family":"Ghaffarifar","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0046-4772","authenticated-orcid":false,"given":"Majid","family":"Pirestani","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5568-1366","authenticated-orcid":false,"given":"Hamidreza","family":"Majidiani","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"18617","reference":[{"key":"10.7774\/cevr.2021.10.1.59_ref1","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1128\/CMR.05013-11","volume":"25","author":"Robert-Gangneux","year":"2012","journal-title":"Clin Microbiol Rev"},{"key":"10.7774\/cevr.2021.10.1.59_ref2","first-page":"153","volume":"6","author":"Ghaffari","year":"2019","journal-title":"J Food Qual Hazards Control"},{"key":"10.7774\/cevr.2021.10.1.59_ref3","doi-asserted-by":"crossref","first-page":"104386","DOI":"10.1016\/j.micpath.2020.104386","volume":"147","author":"Majidiani","year":"2020","journal-title":"Microb Pathog"},{"key":"10.7774\/cevr.2021.10.1.59_ref4","first-page":"289","volume":"29","author":"Buxton","year":"1998","journal-title":"Vet Res"},{"key":"10.7774\/cevr.2021.10.1.59_ref5","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1016\/j.micinf.2005.11.013","volume":"8","author":"Lourenco","year":"2006","journal-title":"Microbes Infect"},{"key":"10.7774\/cevr.2021.10.1.59_ref6","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1007\/s10096-018-03442-6","volume":"38","author":"Dodangeh","year":"2019","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"10.7774\/cevr.2021.10.1.59_ref7","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.amjmed.2004.08.023","volume":"118","author":"Kravetz","year":"2005","journal-title":"Am J Med"},{"key":"10.7774\/cevr.2021.10.1.59_ref8","doi-asserted-by":"crossref","first-page":"104079","DOI":"10.1016\/j.micpath.2020.104079","volume":"142","author":"Ghaffari","year":"2020","journal-title":"Microb Pathog"},{"key":"10.7774\/cevr.2021.10.1.59_ref9","doi-asserted-by":"crossref","first-page":"146","DOI":"10.7774\/cevr.2020.9.2.146","volume":"9","author":"Majidiani","year":"2020","journal-title":"Clin Exp Vaccine Res"},{"key":"10.7774\/cevr.2021.10.1.59_ref10","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1111\/cmi.12426","volume":"17","author":"Morlon-Guyot","year":"2015","journal-title":"Cell Microbiol"},{"key":"10.7774\/cevr.2021.10.1.59_ref11","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1007\/s12639-013-0390-6","volume":"39","author":"Ezz Eldin","year":"2015","journal-title":"J Parasit Dis"},{"key":"10.7774\/cevr.2021.10.1.59_ref12","doi-asserted-by":"crossref","first-page":"2001","DOI":"10.1007\/s00436-012-3047-y","volume":"111","author":"Wu","year":"2012","journal-title":"Parasitol Res"},{"key":"10.7774\/cevr.2021.10.1.59_ref13","doi-asserted-by":"crossref","first-page":"883","DOI":"10.4269\/ajtmh.12-0727","volume":"88","author":"Parthasarathy","year":"2013","journal-title":"Am J Trop Med Hyg"},{"key":"10.7774\/cevr.2021.10.1.59_ref14","doi-asserted-by":"crossref","first-page":"e0143087","DOI":"10.1371\/journal.pone.0143087","volume":"10","author":"Pinzan","year":"2015","journal-title":"PLoS One"},{"key":"10.7774\/cevr.2021.10.1.59_ref15","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1186\/1756-3305-6-163","volume":"6","author":"Macedo","year":"2013","journal-title":"Parasit Vectors"},{"key":"10.7774\/cevr.2021.10.1.59_ref16","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1046\/j.1365-2958.1997.5671913.x","volume":"26","author":"Dobrowolski","year":"1997","journal-title":"Mol Microbiol"},{"key":"10.7774\/cevr.2021.10.1.59_ref17","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1126\/science.1074553","volume":"298","author":"Meissner","year":"2002","journal-title":"Science"},{"key":"10.7774\/cevr.2021.10.1.59_ref18","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.exppara.2017.03.001","volume":"176","author":"Liu","year":"2017","journal-title":"Exp Parasitol"},{"key":"10.7774\/cevr.2021.10.1.59_ref19","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1084\/jem.20041672","volume":"201","author":"Cerede","year":"2005","journal-title":"J Exp Med"},{"key":"10.7774\/cevr.2021.10.1.59_ref20","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1007\/s00436-010-1742-0","volume":"106","author":"Liu","year":"2010","journal-title":"Parasitol Res"},{"key":"10.7774\/cevr.2021.10.1.59_ref21","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.micpath.2019.05.013","volume":"132","author":"Dodangeh","year":"2019","journal-title":"Microb Pathog"},{"key":"10.7774\/cevr.2021.10.1.59_ref22","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1007\/s12639-018-0996-9","volume":"42","author":"Shaddel","year":"2018","journal-title":"J Parasit Dis"},{"key":"10.7774\/cevr.2021.10.1.59_ref23","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1093\/bib\/bbr055","volume":"12","author":"Romano","year":"2011","journal-title":"Brief Bioinform"},{"key":"10.7774\/cevr.2021.10.1.59_ref24","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1007\/s00436-015-4824-1","volume":"115","author":"Wang","year":"2016","journal-title":"Parasitol Res"},{"key":"10.7774\/cevr.2021.10.1.59_ref25","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1016\/j.exppara.2013.09.019","volume":"135","author":"Wang","year":"2013","journal-title":"Exp Parasitol"},{"key":"10.7774\/cevr.2021.10.1.59_ref26","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1385\/1-59259-890-0:571","volume-title":"The proteomics protocols handbook","author":"Gasteiger","year":"2005"},{"key":"10.7774\/cevr.2021.10.1.59_ref27","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1515\/ap-2016-0042","volume":"61","author":"Zhou","year":"2016","journal-title":"Acta Parasitol"},{"key":"10.7774\/cevr.2021.10.1.59_ref28","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.meegid.2018.04.033","volume":"62","author":"Foroutan","year":"2018","journal-title":"Infect Genet Evol"},{"key":"10.7774\/cevr.2021.10.1.59_ref29","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1016\/S0076-6879(96)66034-0","volume":"266","author":"Garnier","year":"1996","journal-title":"Methods Enzymol"},{"key":"10.7774\/cevr.2021.10.1.59_ref30","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1093\/bioinformatics\/16.4.404","volume":"16","author":"McGuffin","year":"2000","journal-title":"Bioinformatics"},{"key":"10.7774\/cevr.2021.10.1.59_ref31","doi-asserted-by":"crossref","first-page":"3991","DOI":"10.1093\/bioinformatics\/btx521","volume":"33","author":"Deleage","year":"2017","journal-title":"Bioinformatics"},{"key":"10.7774\/cevr.2021.10.1.59_ref32","doi-asserted-by":"crossref","first-page":"W230","DOI":"10.1093\/nar\/gki412","volume":"33","author":"Ferre","year":"2005","journal-title":"Nucleic Acids Res"},{"issue":"Suppl 1","key":"10.7774\/cevr.2021.10.1.59_ref33","doi-asserted-by":"crossref","first-page":"S162","DOI":"10.1002\/elps.200900140","volume":"30","author":"Guex","year":"2009","journal-title":"Electrophoresis"},{"key":"10.7774\/cevr.2021.10.1.59_ref34","doi-asserted-by":"crossref","first-page":"1974","DOI":"10.1002\/prot.24086","volume":"80","author":"Park","year":"2012","journal-title":"Proteins"},{"key":"10.7774\/cevr.2021.10.1.59_ref35","doi-asserted-by":"crossref","first-page":"10480","DOI":"10.1038\/s41598-017-09654-8","volume":"7","author":"Bertoni","year":"2017","journal-title":"Sci Rep"},{"key":"10.7774\/cevr.2021.10.1.59_ref36","doi-asserted-by":"crossref","first-page":"W407","DOI":"10.1093\/nar\/gkm290","volume":"35","author":"Wiederstein","year":"2007","journal-title":"Nucleic Acids Res"},{"key":"10.7774\/cevr.2021.10.1.59_ref37","first-page":"197","volume-title":"Artificial immune systems. Proceedings of the International Conference on Artificial Immune Systems; 2004 Sep 13\u201316; Catania, Italy","author":"Saha","year":"2004"},{"key":"10.7774\/cevr.2021.10.1.59_ref38","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1002\/prot.21078","volume":"65","author":"Saha","year":"2006","journal-title":"Proteins"},{"key":"10.7774\/cevr.2021.10.1.59_ref39","doi-asserted-by":"crossref","first-page":"e45152","DOI":"10.1371\/journal.pone.0045152","volume":"7","author":"Yao","year":"2012","journal-title":"PLoS One"},{"key":"10.7774\/cevr.2021.10.1.59_ref40","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/1745-7580-2-2","volume":"2","author":"Larsen","year":"2006","journal-title":"Immunome Res"},{"key":"10.7774\/cevr.2021.10.1.59_ref41","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1186\/1471-2105-9-514","volume":"9","author":"Ponomarenko","year":"2008","journal-title":"BMC Bioinformatics"},{"key":"10.7774\/cevr.2021.10.1.59_ref42","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1186\/1471-2105-11-568","volume":"11","author":"Wang","year":"2010","journal-title":"BMC Bioinformatics"},{"key":"10.7774\/cevr.2021.10.1.59_ref43","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/s00251-011-0579-8","volume":"64","author":"Karosiene","year":"2012","journal-title":"Immunogenetics"},{"key":"10.7774\/cevr.2021.10.1.59_ref44","doi-asserted-by":"crossref","first-page":"4928","DOI":"10.1128\/JVI.02632-06","volume":"81","author":"Kotturi","year":"2007","journal-title":"J Virol"},{"key":"10.7774\/cevr.2021.10.1.59_ref45","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1038\/nbt1215","volume":"24","author":"Moutaftsi","year":"2006","journal-title":"Nat Biotechnol"},{"key":"10.7774\/cevr.2021.10.1.59_ref46","doi-asserted-by":"crossref","first-page":"3195","DOI":"10.1016\/j.vaccine.2004.02.005","volume":"22","author":"Bhasin","year":"2004","journal-title":"Vaccine"},{"key":"10.7774\/cevr.2021.10.1.59_ref47","doi-asserted-by":"crossref","first-page":"e1000048","DOI":"10.1371\/journal.pcbi.1000048","volume":"4","author":"Wang","year":"2008","journal-title":"PLoS Comput Biol"},{"key":"10.7774\/cevr.2021.10.1.59_ref48","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1111\/imm.12889","volume":"154","author":"Jensen","year":"2018","journal-title":"Immunology"},{"key":"10.7774\/cevr.2021.10.1.59_ref49","doi-asserted-by":"crossref","first-page":"2936","DOI":"10.1093\/bioinformatics\/btq551","volume":"26","author":"Magnan","year":"2010","journal-title":"Bioinformatics"},{"key":"10.7774\/cevr.2021.10.1.59_ref50","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/1471-2105-8-4","volume":"8","author":"Doytchinova","year":"2007","journal-title":"BMC Bioinformatics"},{"issue":"Suppl 6","key":"10.7774\/cevr.2021.10.1.59_ref51","doi-asserted-by":"crossref","first-page":"S4","DOI":"10.1186\/1471-2105-14-S6-S4","volume":"14","author":"Dimitrov","year":"2013","journal-title":"BMC Bioinformatics"},{"key":"10.7774\/cevr.2021.10.1.59_ref52","doi-asserted-by":"crossref","first-page":"2200","DOI":"10.1093\/bioinformatics\/btp386","volume":"25","author":"Magnan","year":"2009","journal-title":"Bioinformatics"},{"key":"10.7774\/cevr.2021.10.1.59_ref53","doi-asserted-by":"crossref","first-page":"4119","DOI":"10.1074\/jbc.M008294200","volume":"276","author":"Brecht","year":"2001","journal-title":"J Biol Chem"},{"key":"10.7774\/cevr.2021.10.1.59_ref54","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1136\/vr.129.5.89","volume":"129","author":"Buxton","year":"1991","journal-title":"Vet Rec"},{"key":"10.7774\/cevr.2021.10.1.59_ref55","doi-asserted-by":"crossref","first-page":"S11","DOI":"10.1017\/S003118200000144X","volume":"110 Suppl","author":"Buxton","year":"1995","journal-title":"Parasitology"},{"key":"10.7774\/cevr.2021.10.1.59_ref56","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1586\/erv.10.113","volume":"9","author":"Innes","year":"2010","journal-title":"Expert Rev Vaccines"},{"key":"10.7774\/cevr.2021.10.1.59_ref57","first-page":"235","volume-title":"Fundamental immunology","author":"Berzofsky","year":"1993","edition":"3"},{"key":"10.7774\/cevr.2021.10.1.59_ref58","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1186\/1756-0500-2-111","volume":"2","author":"Lee","year":"2009","journal-title":"BMC Res Notes"},{"key":"10.7774\/cevr.2021.10.1.59_ref59","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1091\/mbc.e12-09-0677","volume":"24","author":"Dephoure","year":"2013","journal-title":"Mol Biol Cell"},{"key":"10.7774\/cevr.2021.10.1.59_ref60","doi-asserted-by":"crossref","first-page":"3773","DOI":"10.1093\/bioinformatics\/btv459","volume":"31","author":"Yang","year":"2015","journal-title":"Bioinformatics"},{"key":"10.7774\/cevr.2021.10.1.59_ref61","first-page":"17","volume":"41","author":"El-Kady","year":"2011","journal-title":"J Egypt Soc Parasitol"},{"key":"10.7774\/cevr.2021.10.1.59_ref62","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1128\/IAI.68.3.1026-1033.2000","volume":"68","author":"Sayles","year":"2000","journal-title":"Infect Immun"},{"key":"10.7774\/cevr.2021.10.1.59_ref63","doi-asserted-by":"crossref","first-page":"104488","DOI":"10.1016\/j.micpath.2020.104488","volume":"149","author":"Nosrati","year":"2020","journal-title":"Microb Pathog"},{"key":"10.7774\/cevr.2021.10.1.59_ref64","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1126\/science.3128869","volume":"240","author":"Suzuki","year":"1988","journal-title":"Science"},{"key":"10.7774\/cevr.2021.10.1.59_ref65","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1007\/978-1-4939-1115-8_19","volume":"1184","author":"Desai","year":"2014","journal-title":"Methods Mol Biol"},{"key":"10.7774\/cevr.2021.10.1.59_ref66","doi-asserted-by":"crossref","first-page":"e1002829","DOI":"10.1371\/journal.pcbi.1002829","volume":"8","author":"Kringelum","year":"2012","journal-title":"PLoS Comput Biol"},{"issue":"Suppl 1","key":"10.7774\/cevr.2021.10.1.59_ref67","doi-asserted-by":"crossref","first-page":"S6","DOI":"10.1186\/1472-6807-10-S1-S6","volume":"10","author":"Zhao","year":"2010","journal-title":"BMC Struct Biol"},{"key":"10.7774\/cevr.2021.10.1.59_ref68","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1080\/20477724.2018.1446773","volume":"112","author":"Kazi","year":"2018","journal-title":"Pathog Glob Health"},{"key":"10.7774\/cevr.2021.10.1.59_ref69","doi-asserted-by":"crossref","first-page":"e00711","DOI":"10.1128\/mSphere.00711-18","volume":"4","author":"Saraav","year":"2019","journal-title":"mSphere"},{"key":"10.7774\/cevr.2021.10.1.59_ref70","doi-asserted-by":"crossref","first-page":"e1007871","DOI":"10.1371\/journal.ppat.1007871","volume":"15","author":"Sardinha-Silva","year":"2019","journal-title":"PLoS Pathog"}],"container-title":["Clinical and Experimental Vaccine Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/ecevr.org\/pdf\/10.7774\/cevr.2021.10.1.59","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ecevr.org\/DOIx.php?id=10.7774\/cevr.2021.10.1.59","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ecevr.org\/DOIx.php?id=10.7774\/cevr.2021.10.1.59","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T10:55:11Z","timestamp":1697885711000},"score":1,"resource":{"primary":{"URL":"https:\/\/ecevr.org\/DOIx.php?id=10.7774\/cevr.2021.10.1.59"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021]]},"references-count":70,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021]]}},"URL":"https:\/\/doi.org\/10.7774\/cevr.2021.10.1.59","relation":{},"ISSN":["2287-3651","2287-366X"],"issn-type":[{"value":"2287-3651","type":"print"},{"value":"2287-366X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021]]},"assertion":[{"value":"2020-10-06","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-25","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-31","name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication History"}},{"value":"\u00a9 Korean Vaccine Society.","name":"copyright","label":"Copyright","group":{"name":"Copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.","name":"license","label":"License","explanation":{"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},"group":{"name":"Copyright_and_licensing","label":"Copyright and Licensing"}}]}}