{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T01:39:43Z","timestamp":1780969183405,"version":"3.54.1"},"reference-count":0,"publisher":"JCDR Research and Publications","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/jcdr.net\/article_fulltext.asp?issn=0973-709x&year=2015&volume=9&issue=4&page=DC01&issn=0973-709x&id=5797"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCDR"],"published-print":{"date-parts":[[2015,4,1]]},"abstract":"<jats:p>Introduction: Klebsiella pneumoniae is one of the main oppor tunistic pathogens which can cause different types of infections. Production of betalactamases like AmpC and ESBL mostly lead to betalactam resistance in these GramNegative bacteria.\n\nThe aim of this study was the detection of AmpCproducing K. pneumoniae in clinical isolates.\n\nMaterials and Methods: Three hundred and three isolates of K. pneumoniae were identified. Double disc method including cefoxitin with cefepime and using boronic acid with cloxacillin were performed as two phenotypic methods for detection of AmpC. Amplification of AmpC gene was performed by PCR.\n\nResults: Eight and three isolates showed positive results in double disc method and by using boronic acid with cloxacillin, respectively. Five isolates had specific band for AmpC gene after electrophoresis.\n\nConclusion: Our results were indicated the low prevalence of AmpCproducer K. pnemoniae in Iran. On the other hand these two tested phenotypic methods showed low sensitivity for detection of AmpC<\/jats:p>","DOI":"10.7860\/jcdr\/2015\/11037.5797","type":"journal-article","created":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T05:00:11Z","timestamp":1779080411000},"source":"Crossref","is-referenced-by-count":1,"title":["Phenotypic and Molecular Characterization of Plasmid Mediated AmpC among Clinical Isolates of Klebsiella pneumoniae Isolated from Different Hospitals in Tehran"],"prefix":"10.7860","author":[{"given":"Leila","family":"Azimi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gholamreza","family":"Erajiyan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malihe","family":"Talebi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parviz","family":"Owlia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahsa","family":"Bina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Shojaie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdolaziz Rastegar","family":"Lari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4601","published-online":{"date-parts":[[2015,4,1]]},"container-title":["JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH"],"original-title":[],"link":[{"URL":"https:\/\/jcdr.net\/article_fulltext.asp?issn=0973-709x&year=2015&volume=9&issue=4&page=DC01&issn=0973-709x&id=5797","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T05:00:12Z","timestamp":1779080412000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcdr.net\/article_fulltext.asp?issn=0973-709x&year=2015&volume=9&issue=4&page=DC01&issn=0973-709x&id=5797"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4,1]]},"references-count":0,"URL":"https:\/\/doi.org\/10.7860\/jcdr\/2015\/11037.5797","relation":{},"ISSN":["2249-782X","0973-709X"],"issn-type":[{"value":"2249-782X","type":"print"},{"value":"0973-709X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4,1]]}}}