{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T22:32:08Z","timestamp":1793658728817,"version":"4.5.1"},"reference-count":17,"publisher":"Editorial Committee of Japanese Journal of Infectious Diseases, National Institute of Infectious Dis","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Jpn J Infect Dis"],"published-print":{"date-parts":[[2021,9,30]]},"DOI":"10.7883\/yoken.jjid.2020.1079","type":"journal-article","created":{"date-parts":[[2021,2,25]],"date-time":"2021-02-25T22:09:34Z","timestamp":1614290974000},"page":"465-472","source":"Crossref","is-referenced-by-count":12,"title":["Performance Evaluation of Real-Time RT-PCR Assays for the Detection of Severe Acute Respiratory Syndrome Coronavirus-2 Developed by the National Institute of Infectious Diseases, Japan"],"prefix":"10.7883","volume":"74","author":[{"given":"Kazuya","family":"Shirato","sequence":"first","affiliation":[{"name":"Department of Virology III, National Institute of Infectious Diseases, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuriko","family":"Tomita","sequence":"additional","affiliation":[{"name":"Department of Virology III, National Institute of Infectious Diseases, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Katoh","sequence":"additional","affiliation":[{"name":"Department of Virology III, National Institute of Infectious Diseases, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Souichi","family":"Yamada","sequence":"additional","affiliation":[{"name":"Department of Virology I, National Institute of Infectious Diseases, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuetsu","family":"Fukushi","sequence":"additional","affiliation":[{"name":"Department of Virology I, National Institute of Infectious Diseases, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shutoku","family":"Matsuyama","sequence":"additional","affiliation":[{"name":"Department of Virology III, National Institute of Infectious Diseases, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Takeda","sequence":"additional","affiliation":[{"name":"Department of Virology III, National Institute of Infectious Diseases, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4631","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"1. Wu F, Zhao S, Yu B, et al. A new coronavirus associated with human respiratory disease in China. Nature. 2020;579:265-9.","DOI":"10.1038\/s41586-020-2008-3"},{"key":"2","doi-asserted-by":"crossref","unstructured":"2. Xiao K, Zhai J, Feng Y, et al. Isolation of SARS-CoV-2-related coronavirus from Malayan pangolins. Nature. 2020;583:286-9.","DOI":"10.1038\/s41586-020-2313-x"},{"key":"3","unstructured":"3. World Health Organization (WHO). WHO Coronavirus (COVID-19) Dashboard. Available at &lt;https:\/\/covid19.who.int\/&gt;. Accessed October 21, 2020."},{"key":"4","unstructured":"4. World Health Organization (WHO). Coronavirus disease (COVID-19) Weekly Epidemiological Update and Weekly Operational Update. Available at: &lt;https:\/\/www.who.int\/emergencies\/diseases\/novel-coronavirus-2019\/situation-reports\/&gt;. Accessed December 8, 2020."},{"key":"5","doi-asserted-by":"crossref","unstructured":"5. Shirato K, Nao N, Katano H, et al. Development of genetic diagnostic methods for detection for novel coronavirus 2019(nCoV-2019) in Japan. Jpn J Infect Dis. 2020;73:304-7.","DOI":"10.7883\/yoken.JJID.2020.061"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6. Corman VM, Landt O, Kaiser M, et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020;25:2000045.","DOI":"10.2807\/1560-7917.ES.2020.25.3.2000045"},{"key":"7","unstructured":"7. Corman V, Bleicker T, Br\u00fcnink S, et al. Diagnostic detection of 2019-nCoV by real-time RT-PCR. Available at &lt;https:\/\/www.who.int\/docs\/default-source\/coronaviruse\/protocol-v2-1.pdf&gt;. Accessed December 8 2020."},{"key":"8","doi-asserted-by":"crossref","unstructured":"8. Shirato K, Nao N, Matsuyama S, et al. An ultra-rapid real-time RT-PCR method using the PCR1100 to detect severe acute respiratory syndrome coronavirus-2. Jpn J Infect Dis. 2021;74:29-34.","DOI":"10.7883\/yoken.JJID.2020.324"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9. Matsuyama S, Nao N, Shirato K, et al. Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells. Proc Natl Acad Sci U S A. 2020;117:7001-3.","DOI":"10.1073\/pnas.2002589117"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10. Nao N, Sato K, Yamagishi J, et al. Consensus and variations in cell line specificity among human metapneumovirus strains. PLoS One. 2019;14:e0215822.","DOI":"10.1371\/journal.pone.0215822"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11. Sekizuka T, Itokawa K, Hashino M, et al. A genome epidemiological study of SARS-CoV-2 introduction into Japan. mSphere. 2020;5:e00786-20.","DOI":"10.1128\/mSphere.00786-20"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12. Shirato K, Kawase M, Watanabe O, et al. Differences in neutralizing antigenicity between laboratory and clinical isolates of HCoV-229E isolated in Japan in 2004-2008 depend on the S1 region sequence of the spike protein. J Gen Virol. 2012;93:1908-17.","DOI":"10.1099\/vir.0.043117-0"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13. Shirogane Y, Takeda M, Iwasaki M, et al. Efficient multiplication of human metapneumovirus in Vero cells expressing the transmembrane serine protease TMPRSS2. J Virol. 2008;82:8942-6.","DOI":"10.1128\/JVI.00676-08"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14. Shirato K, Kawase M, Matsuyama S. Wild-type human coronaviruses prefer cell-surface TMPRSS2 to endosomal cathepsins for cell entry. Virology. 2018;517:9-15.","DOI":"10.1016\/j.virol.2017.11.012"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15. Kaida A, Kubo H, Takakura K, et al. Associations between co-detected respiratory viruses in children with acute respiratory infections. Jpn J Infect Dis. 2014;67:469-75.","DOI":"10.7883\/yoken.67.469"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16. Shirato K, Nao N, Kawase M, et al. An ultra-rapid real-time RT-PCR method using PCR1100 for detecting human orthopneumovirus. Jpn J Infect Dis. 2020;73:465-8.","DOI":"10.7883\/yoken.JJID.2020.182"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17. Katoh K, Rozewicki J, Yamada KD. MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Brief Bioinform. 2019;20:1160-6.","DOI":"10.1093\/bib\/bbx108"}],"container-title":["Japanese Journal of Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/yoken\/74\/5\/74_JJID.2020.1079\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,25]],"date-time":"2021-09-25T03:50:06Z","timestamp":1632541806000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/yoken\/74\/5\/74_JJID.2020.1079\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,30]]},"references-count":17,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021]]}},"URL":"https:\/\/doi.org\/10.7883\/yoken.jjid.2020.1079","relation":{},"ISSN":["1344-6304","1884-2836"],"issn-type":[{"value":"1344-6304","type":"print"},{"value":"1884-2836","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,30]]},"article-number":"JJID.2020.1079"}}