{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T11:14:03Z","timestamp":1779102843212,"version":"3.51.4"},"reference-count":11,"publisher":"Editorial Committee of Japanese Journal of Infectious Diseases, National Institute of Infectious Dis","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Jpn J Infect Dis"],"published-print":{"date-parts":[[2022,5,31]]},"DOI":"10.7883\/yoken.jjid.2021.416","type":"journal-article","created":{"date-parts":[[2021,9,29]],"date-time":"2021-09-29T22:10:02Z","timestamp":1632953402000},"page":"309-313","source":"Crossref","is-referenced-by-count":1,"title":["Negative Results of Nucleic Acid Amplification Tests for SARS-CoV-2 in Clinical Practice May Vary among Six Molecular Assays in Patients with COVID-19"],"prefix":"10.7883","volume":"75","author":[{"given":"Ayano","family":"Motohashi","sequence":"first","affiliation":[{"name":"Clinical Laboratory Department, National Center for Global Health and Medicine, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kei","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Disease Control and Prevention Center, National Center for Global Health and Medicine, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhisa","family":"Mezaki","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Department, National Center for Global Health and Medicine, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ataru","family":"Moriya","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Department, National Center for Global Health and Medicine, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masami","family":"Kurokawa","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Department, National Center for Global Health and Medicine, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Oki","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Department, National Center for Global Health and Medicine, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Honami","family":"Ando","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Department, National Center for Global Health and Medicine, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erina","family":"Isaka","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Department, National Center for Global Health and Medicine, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayaka","family":"Usami","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Department, National Center for Global Health and Medicine, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Ide","sequence":"additional","affiliation":[{"name":"Disease Control and Prevention Center, National Center for Global Health and Medicine, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiji","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Disease Control and Prevention Center, National Center for Global Health and Medicine, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takato","family":"Nakamoto","sequence":"additional","affiliation":[{"name":"Disease Control and Prevention Center, National Center for Global Health and Medicine, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidetoshi","family":"Nomoto","sequence":"additional","affiliation":[{"name":"Disease Control and Prevention Center, National Center for Global Health and Medicine, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norio","family":"Ohmagari","sequence":"additional","affiliation":[{"name":"Disease Control and Prevention Center, National Center for Global Health and Medicine, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4631","reference":[{"key":"1","unstructured":"1. National Institute of Infectious Diseases and Tuberculosis and Infectious Diseases Control Division, Ministry of Health, Labour and Welfare, Japan. Validation of 2019-nCoV gene testing method in which clinical evaluation results were obtained using clinical specimens. Available at &lt;https:\/\/www.niid.go.jp\/niid\/images\/lab-manual\/2019-nCoV-17-current.pdf&gt;. Accessed April 30, 2021. Japanese."},{"key":"2","unstructured":"2. National Institute of Infectious Diseases. Evaluation of performance of genetic testing for new coronavirus (2019-nCoV). Available at &lt;https:\/\/www.niid.go.jp\/niid\/ja\/diseases\/ka\/corona-virus\/2019-ncov\/9482-covid14-15.html&gt;. Accessed April 30, 2021. Japanese."},{"key":"3","doi-asserted-by":"crossref","unstructured":"3. Lieberman JA, Pepper G, Naccache SN, et al. Comparison of commercially available and laboratory-developed tests for in vitro detection of SARS-CoV-2 in clinical laboratories. J Clin Microbiol. 2020;58:e00821-20.","DOI":"10.1128\/JCM.00821-20"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4. Lowe CF, Matic N, Ritchie G, et al. Detection of low levels of SARS\u2010CoV\u20102 RNA from nasopharyngeal swabs using three commercial molecular assays. J Clin Virol. 2020;128:104387.","DOI":"10.1016\/j.jcv.2020.104387"},{"key":"5","unstructured":"5. National Institute of Infectious Diseases. The manual for the detection of pathogen 2019-nCoV Ver. 2.9.1. Available at &lt;https:\/\/www.niid.go.jp\/niid\/images\/lab-manual\/2019-nCoV20200319.pdf&gt;. Accessed April 30, 2021. Japanese."},{"key":"6","doi-asserted-by":"crossref","unstructured":"6. Landis JR, Koch GG. The measurement of observer agreement for categorical data. Biometrics. 1977;33:159-174.","DOI":"10.2307\/2529310"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7. Tsutae W, Chaochaisit W, Aoshima H, et al. Detecting and isolating false negatives of SARS-CoV-2 primers and probe sets among the Japanese Population: a laboratory testing methodology and study. medRxiv. 2020. 10.1101\/2020.10.07.20208264. Preprint.","DOI":"10.1101\/2020.10.07.20208264"},{"key":"8","doi-asserted-by":"crossref","unstructured":"8. Moran A, Beavis KG, Matushek SM, et al. Detection of SARS-CoV-2 by use of the Cepheid Xpert Xpress SARS-CoV-2 and Roche cobas SARS-CoV-2 assays. J Clin Microbiol. 2020;58:e00772-20.","DOI":"10.1128\/JCM.00772-20"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9. Shi D, Wu W, Wang Q, et al. Clinical characteristics and factors associated with long-term viral excretion in patients with severe acute respiratory syndrome coronavirus 2 infection: a single-center 28-day study. J Infect Dis. 2020;222:910-918.","DOI":"10.1093\/infdis\/jiaa388"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10. Li L, Li X, Guo Z, et al. Influence of storage conditions on SARS-CoV-2 nucleic acid detection in throat swabs. J Infect Dis. 2020;222:203-205.","DOI":"10.1093\/infdis\/jiaa272"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11. Rhee C, Kanjilal S, Baker M, et al. Duration of SARS-CoV-2 infectivity: when is it safe to discontinue isolation? Clin Infect Dis. 2020;72:1467-1474.","DOI":"10.1093\/cid\/ciaa1249"}],"container-title":["Japanese Journal of Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/yoken\/75\/3\/75_JJID.2021.416\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,28]],"date-time":"2022-05-28T04:22:26Z","timestamp":1653711746000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/yoken\/75\/3\/75_JJID.2021.416\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,31]]},"references-count":11,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022]]}},"URL":"https:\/\/doi.org\/10.7883\/yoken.jjid.2021.416","relation":{},"ISSN":["1344-6304","1884-2836"],"issn-type":[{"value":"1344-6304","type":"print"},{"value":"1884-2836","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,31]]},"article-number":"JJID.2021.416"}}