{"status":"ok","message-type":"work-list","message-version":"1.0.0","message":{"facets":{},"total-results":21554,"items":[{"institution":[{"name":"Research Square"}],"indexed":{"date-parts":[[2024,1,21]],"date-time":"2024-01-21T06:50:17Z","timestamp":1705819817193},"posted":{"date-parts":[[2024,1,19]]},"group-title":"In Review","reference-count":99,"publisher":"Research Square Platform LLC","license":[{"start":{"date-parts":[[2024,1,19]],"date-time":"2024-01-19T00:00:00Z","timestamp":1705622400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"accepted":{"date-parts":[[2024,1,5]]},"abstract":"Abstract<\/jats:title>\n Background\n Trombiculid mites are globally distributed, highly diverse arachnids that largely lack molecular resources such as whole mitogenomes for the elucidation of taxonomic relationships. Trombiculid larvae (chiggers) parasitise vertebrates and can transmit bacteria (Orientia<\/jats:italic> spp.) responsible for scrub typhus, a zoonotic febrile illness. Orientia tsutsugamushi<\/jats:italic> causes most cases of scrub typhus and is endemic to the Asia-Pacific Region, where it is transmitted by Leptotrombidium<\/jats:italic> spp. chiggers. However, in Dubai, Candidatus<\/jats:italic> Orientia chuto was isolated from a case of scrub typhus and is also known to circulate among rodents in Saudi Arabia and Kenya, although its vectors remain poorly defined. In addition to Orientia<\/jats:italic>, chiggers are often infected with other potential pathogens or arthropod-specific endosymbionts, but their significance for trombiculid biology and public health is unclear.\nResults\n Pooled chiggers of 10 species were collected from rodents in southwestern Saudi Arabia and screened for Orientia<\/jats:italic> DNA by PCR. Two species (Microtrombicula muhaylensis<\/jats:italic> and Pentidionis agamae<\/jats:italic>) produced positive results for the htrA<\/jats:italic> gene, although Ca<\/jats:italic>. Orientia chuto DNA was confirmed by Sanger sequencing only in P. agamae<\/jats:italic>. Metagenomic sequencing of three pools of P. agamae<\/jats:italic> provided evidence for two other bacterial associates: a spirochaete and a Wolbachia<\/jats:italic> symbiont. Phylogenetic analysis of 16S rRNA and multi-locus sequence typing genes placed the spirochaete in a clade of micromammal-associated Borrelia<\/jats:italic> spp. that are widely-distributed globally with no known vector. For the Wolbachia<\/jats:italic> symbiont, a genome assembly was obtained that allowed phylogenetic localisation in a novel, divergent clade. Cytochrome c oxidase I (coi<\/jats:italic>) gene barcodes for Saudi Arabian chiggers enabled comparisons with global chigger diversity, revealing several cases of discordance with classical taxonomy. Complete mitogenome assemblies were obtained for the three P. agamae<\/jats:italic> pools and almost 50 SNPs were identified, despite a common geographic origin.\nConclusions\n P. agamae<\/jats:italic> was identified as a potential vector of Ca.<\/jats:italic> Orientia chuto on the Arabian Peninsula. The detection of an unusual Borrelia<\/jats:italic> sp. and a divergent Wolbachia<\/jats:italic> symbiont in P. agamae<\/jats:italic> indicated links with chigger microbiomes in other parts of the world, while coi<\/jats:italic> barcoding and mitogenomic analyses greatly extended our understanding of inter- and intraspecific relationships in trombiculid mites.<\/jats:p>","DOI":"10.21203\/rs.3.rs-3837555\/v1","type":"posted-content","created":{"date-parts":[[2024,1,19]],"date-time":"2024-01-19T20:53:47Z","timestamp":1705697627000},"source":"Crossref","is-referenced-by-count":0,"title":["Microbiome and mitogenomics of the chigger mite Pentidionis agamae: Potential role as an Orientia vector and associations with divergent clades of Wolbachia and Borrelia"],"prefix":"10.21203","author":[{"given":"Hadil A.","family":"Alkathiry","sequence":"first","affiliation":[{"name":"Imam Mohammad Ibn Saud Islamic University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samia Q.","family":"Alghamdi","sequence":"additional","affiliation":[{"name":"Al-Baha University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amit","family":"Sinha","sequence":"additional","affiliation":[{"name":"New England Biolabs (United States)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriele","family":"Margos","sequence":"additional","affiliation":[{"name":"Bavarian Health and Food Safety Authority"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandr A.","family":"Stekolnikov","sequence":"additional","affiliation":[{"name":"Zoological Institute of the Russian Academy of Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdulaziz N.","family":"Alagaili","sequence":"additional","affiliation":[{"name":"King Saud University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alistair C.","family":"Darby","sequence":"additional","affiliation":[{"name":"University of Liverpool"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin L.","family":"Makepeace","sequence":"additional","affiliation":[{"name":"University of Liverpool"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing Jing","family":"Khoo","sequence":"additional","affiliation":[{"name":"University of Liverpool"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"8761","reference":[{"key":"ref1","first-page":"1","article-title":"The scrub-typhus and scrub-itch mites (Trombiculidae, Acarina) of the Asiatic-Pacific region","volume":"10","author":"Womersley H","year":"1952","unstructured":"Womersley H. 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Trombiculid larvae (chiggers) parasitise vertebrates and can transmit bacteria (Orientia<\/jats:italic> spp.) responsible for scrub typhus, a zoonotic febrile illness. Orientia tsutsugamushi<\/jats:italic> causes most cases of scrub typhus and is endemic to the Asia-Pacific Region, where it is transmitted by Leptotrombidium<\/jats:italic> spp. chiggers. However, in Dubai, Candidatus<\/jats:italic> Orientia chuto was isolated from a case of scrub typhus and is also known to circulate among rodents in Saudi Arabia and Kenya, although its vectors remain poorly defined. In addition to Orientia<\/jats:italic>, chiggers are often infected with other potential pathogens or arthropod-specific endosymbionts, but their significance for trombiculid biology and public health is unclear.<\/jats:p>\n <\/jats:sec>\n Results<\/jats:title>\n Ten chigger species were collected from rodents in southwestern Saudi Arabia. Chiggers were pooled according to species and screened for Orientia<\/jats:italic> DNA by PCR. Two species (Microtrombicula muhaylensis<\/jats:italic> and Pentidionis agamae<\/jats:italic>) produced positive results for the htrA<\/jats:italic> gene, although Ca<\/jats:italic>. Orientia chuto DNA was confirmed by Sanger sequencing only in P. agamae<\/jats:italic>. Metagenomic sequencing of three pools of P. agamae<\/jats:italic> provided evidence for two other bacterial associates: a spirochaete and a Wolbachia<\/jats:italic> symbiont. Phylogenetic analysis of 16S rRNA and multi-locus sequence typing genes placed the spirochaete in a clade of micromammal-associated Borrelia<\/jats:italic> spp. that are widely-distributed globally with no known vector. For the Wolbachia<\/jats:italic> symbiont, a genome assembly was obtained that allowed phylogenetic localisation in a novel, divergent clade. Cytochrome c oxidase I (COI<\/jats:italic>) barcodes for Saudi Arabian chiggers enabled comparisons with global chigger diversity, revealing several cases of discordance with classical taxonomy. Complete mitogenome assemblies were obtained for the three P. agamae<\/jats:italic> pools and almost 50 SNPs were identified, despite a common geographic origin.<\/jats:p>\n <\/jats:sec>\n Conclusions<\/jats:title>\n P. agamae<\/jats:italic> was identified as a potential vector of Ca.<\/jats:italic> Orientia chuto on the Arabian Peninsula. The detection of an unusual Borrelia<\/jats:italic> sp. and a divergent Wolbachia<\/jats:italic> symbiont in P. agamae<\/jats:italic> indicated links with chigger microbiomes in other parts of the world, while COI<\/jats:italic> barcoding and mitogenomic analyses greatly extended our understanding of inter- and intraspecific relationships in trombiculid mites.<\/jats:p>\n <\/jats:sec>","DOI":"10.1186\/s12864-024-10301-6","type":"journal-article","created":{"date-parts":[[2024,4,17]],"date-time":"2024-04-17T12:03:07Z","timestamp":1713355387000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Microbiome and mitogenomics of the chigger mite Pentidionis agamae: potential role as an Orientia vector and associations with divergent clades of Wolbachia and Borrelia"],"prefix":"10.1186","volume":"25","author":[{"given":"Hadil A.","family":"Alkathiry","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samia Q.","family":"Alghamdi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Sinha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Margos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandr A.","family":"Stekolnikov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulaziz N.","family":"Alagaili","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alistair C.","family":"Darby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin L.","family":"Makepeace","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing Jing","family":"Khoo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,4,17]]},"reference":[{"key":"10301_CR1","first-page":"1","volume":"10","author":"H Womersley","year":"1952","unstructured":"Womersley H. 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Scabies is an eight-legged mite which belongs to the phylogenetic class of arachnids, which also includes spiders and scorpions. More generally it is an arthropod, an invertebrate with an exoskeleton encompassing a large number of species including six-legged insects as well as crustaceans better known to humans as food in the forms of lobsters, crab, and shrimp, and the like. And most generically, it belongs to the animal kingdom. This chapter describes the physical characteristics and life cycle of scabies which varies according to the sex of the mite. The female scabies mite burrows into the skin where she lays her eggs, and constantly moves forward to seek nutrients while simultaneously evading the host immune system. The mating habits of scabies are discussed as well as behavior of the mite when present in warm and humid (tropical), climates.<\/p>","DOI":"10.1093\/med\/9780192848406.003.0004","type":"book-chapter","created":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T13:24:13Z","timestamp":1653657853000},"page":"55-72","source":"Crossref","is-referenced-by-count":0,"title":["The Mite"],"prefix":"10.1093","author":[{"given":"Errol","family":"Craig","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,5]]},"container-title":["The Itch"],"original-title":["The Mite"],"language":"en","deposited":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T21:40:52Z","timestamp":1659476452000},"score":14.494137,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/book\/42074\/chapter\/355934256"}},"issued":{"date-parts":[[2022,5]]},"ISBN":["9780192848406","9780191943720"],"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/med\/9780192848406.003.0004","published":{"date-parts":[[2022,5]]}},{"indexed":{"date-parts":[[2022,4,2]],"date-time":"2022-04-02T05:09:07Z","timestamp":1648876147773},"reference-count":0,"publisher":"Scientific Societies","content-domain":{"domain":[],"crossmark-restriction":false},"published-print":{"date-parts":[[2013,3,1]]},"DOI":"10.1094\/grow-cot-03-13-009","type":"dataset","created":{"date-parts":[[2020,8,13]],"date-time":"2020-08-13T22:12:40Z","timestamp":1597356760000},"source":"Crossref","is-referenced-by-count":0,"title":["Terminating Spider Mite Applications in Cotton"],"prefix":"10.1094","member":"1665","container-title":["Grow: Plant Health Exchange"],"original-title":["Terminating Spider Mite Applications in Cotton"],"deposited":{"date-parts":[[2020,8,13]],"date-time":"2020-08-13T22:12:42Z","timestamp":1597356762000},"score":14.490658,"resource":{"primary":{"URL":"http:\/\/planthealthexchange.org\/cotton\/Pages\/GROW-COT-03-13-009.aspx"}},"issued":{"date-parts":[[2013,3,1]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1094\/grow-cot-03-13-009","published":{"date-parts":[[2013,3,1]]}}],"items-per-page":15,"query":{"start-index":0,"search-terms":"\"Pentidionis\" mite"}}}