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Among the materials used for root-end fillings, Mineral Trioxide Aggregate (MTA) and Glass Ionomer Cement (GIC) are frequently employed due to their biological and mechanical properties. However, their sealing capabilities remain a topic of debate.\nAim: The present ex vivo study aimed to compare the apical sealing ability of MTA and GIC using dye penetration analysis and stereomicroscopy.Materials and Methods: Thirty extracted single-rooted human teeth were prepared and divided into two groups (n=15). Apical plugs of 3 mm were placed using ProRoot MTA and conventional GIC. After 72 hours of setting, samples were immersed in 2% methylene blue for 48 hours. Dye penetration was measured under 25\u00d7 stereomicroscopy. Statistical analysis was conducted using the Mann-Whitney U test (p   0.05).\nResults: MTA exhibited a mean dye penetration score of 0.30 \u00b1 0.46, while GIC showed a higher mean score of 0.57 \u00b1 0.50. The difference between the two groups was statistically significant (p = 0.039), indicating that MTA provided a more effective apical seal under the conditions of this ex vivo study. Moreover, MTA samples showed less variability in leakage scores compared to GIC.Conclusion: Within the limitations of this ex vivo study, GIC showed better apical sealing ability than MTA under the tested conditions. Nevertheless, MTA remains a reliable and well-established material in endodontic practice. 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Treatment of tooth discoloration after the use of MTA. Aust Dent J. 2011;56(4):457-461."},{"key":"ref10","unstructured":"11. D'Addazio G, et al. Microscopic evaluation of apical sealing ability of calcium silicate-based sealers: A comparative study. Int Endod J. 2017;50(4):369-378."},{"key":"ref11","unstructured":"12. Nicholson JW. Chemistry of glass ionomer cements: A review. Biomaterials. 2006;27(17):2901-2908."},{"key":"ref12","unstructured":"13. Mickenautsch S, Yengopal V, Leal SC. Fluoride release of glass ionomers and caries prevention: A meta-analysis. J Dent. 2011;39(9):601-612."},{"key":"ref13","unstructured":"14. Yap AU, Pek YS, Cheang PH, Khor KA. Moisture sensitivity of GICs and effects on sealing. Oper Dent. 2003;28(6):672-678."},{"key":"ref14","unstructured":"15. Bukhari S, Alenazy MS, Alshagroud RS. Marginal adaptation of MTA and GIC: An SEM study. Saudi Endod J. 2017;7(1):6-12."},{"key":"ref15","unstructured":"16. Atmeh AR, Chong EZ, Richard G, Festy F, Watson TF. Fracture resistance of GICs compared to other endodontic materials. Dent Mater. 2012;28(5):529-536."},{"key":"ref16","unstructured":"17. Kaur R, et al. Assessment of apical seal in endodontics using CLSM. J Funct Biomater. 2022;13(2):341."},{"key":"ref17","unstructured":"18. Borges RP, Sousa-Neto MD, Versiani MA, et al. CLSM analysis of sealer penetration. J Appl Oral Sci. 2021;29:e20210130."},{"key":"ref18","unstructured":"19. Grech L, Mallia B, Camilleri J. Properties of Biodentine: A literature review. J Appl Biomater Funct Mater. 2013;11(3):e115-e119."},{"key":"ref19","unstructured":"20. Antunes HS, Gominho LF, Silva JA, et al. Sealing ability of EndoSequence Root Repair Material and MTA. 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