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The structure of the feed and the quality of mixing are determined by means of the Pennsylvania sorting system, the level of feed on the sieves is in line with the normative. The results of the production experiment on feeding dairy cows with Actisaf Sc 47, which has a probiotic effect, indicate better digestibility of the feed when the additive is introduced, the use of the drug contributes to: reducing ketone bodies in milk, the beneficial effect of improved feeding, by including yeast on the health of animals, improving the health of the udder, manifested in a decrease in somatic cells in milk. The milk yield with the use of the additive is increased, the milk protein is also increased, since its absorption in the rumen is improved. With the use of additives, the appetite and eatability of the feed improves, so in the group before feeding the probiotic, the percentage of feed residue on the feed table is higher than in the experiment. Dry matter intake is 4.32% higher when feeding supplements, while feed conversion is increased. Saving feed costs per 1 kg of milk is 0.97 rubles. 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Groups 1, 2, 3 and 4 were fed with diets A, B, C and D respectively and their fasting blood glucose level were determined at intervals for a period of twenty-one days. Group 3 had the lowest blood glucose level range (80 to 100 mg\/dL) more than diets A and B which has blood glucose level range of 82 to 109 mg\/dL and 103 to 124 mg\/dL respectively. This is an indication that the particle size of diet C is the best in the management of diabetes among the other diets. Therefore, the particle size of diet C is more effective in the management of diabetes than that of diets A and B. 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Directorate for Geosciences","doi-asserted-by":"publisher","award":["1559002"],"award-info":[{"award-number":["1559002"]}],"id":[{"id":"10.13039\/100000085","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000155","name":"NSF | BIO | Division of Environmental Biology","doi-asserted-by":"publisher","award":["1457160"],"award-info":[{"award-number":["1457160"]}],"id":[{"id":"10.13039\/100000155","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006206","name":"DOE | SC | Biological and Environmental Research","doi-asserted-by":"publisher","award":["SC0016410"],"award-info":[{"award-number":["SC0016410"]}],"id":[{"id":"10.13039\/100006206","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"published-print":{"date-parts":[[2019,8,1]]},"abstract":"Abstract<\/jats:title>\n The paradigm that only 1% of microbes are culturable has had a profound impact on our understanding of microbial ecology and is still a major motivation for mostly using molecular tools to characterize microbial communities. However, this point is often expressed vaguely, suggesting that some scientists have different interpretations of the paradigm. In addition, there have been substantial advances in cultivation techniques suggesting that this paradigm may no longer be correct. To quantify bacterial culturability across six major biomes, I found that the median 16S rRNA similarity of bacteria to known cultured relatives was 97.3\u2009\u00b1\u20092.3% (s.d.). Furthermore, 52.0\u2009\u00b1\u200924% of sequences and 34.9\u2009\u00b1\u200923% of taxa (defined as &gt;97% similar) had a closely related cultured relative. 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